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目的观察成年大鼠心室肌细胞调节性细胞容积减小(regulatory volume decrease,RVD)的过程,探讨参与RVD过程的离子通道机制。方法将急性分离的大鼠心室肌细胞放入低渗溶液中,利用细胞体积测量系统测定细胞平均容积的变化过程和离子通道的参与过程。结果大鼠心室肌细胞具有良好的RVD功能;该过程可被氯通道阻断剂如蒽-9-羧酸(anthracene-9-carboxylic acid,9-AC,500μmol/L)和钾通道阻断剂CsCl(5mmol/L)所阻断。进一步的研究发现,ATP敏感钾通道(KATP)的阻断剂格列苯脲(glibenclamide,10μmol/L)可以明显地抑制细胞的RVD过程。结论成年大鼠心室肌细胞具有RVD功能,RVD过程的完成有赖于氯通道和钾通道的平行激活,ATP敏感钾通道是参与容积调节的钾通道之一。
Objective To observe the process of regulatory volume decrease (RVD) in adult rat ventricular myocytes and to explore the mechanism of ion channels involved in RVD. Methods Acutely isolated rat ventricular myocytes were placed in hypotonic solution, and the changes of cell mean volume and ion channels were measured by cell volume measurement system. Results Rat ventricular myocytes had good RVD function, which could be blocked by chloride channel blockers such as anthracene-9-carboxylic acid (9-AC, 500μmol / L) and potassium channel blockers CsCl (5mmol / L) blocked. Further studies found that glibenclamide (10 μmol / L), a blocker of ATP-sensitive potassium channel (KATP), significantly inhibited the RVD of cells. Conclusion Adult rat ventricular myocytes have RVD function. The completion of RVD depends on the parallel activation of chloride channel and potassium channel. ATP-sensitive potassium channel is one of the potassium channels involved in volume regulation.