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目的:探讨巴戟天萃取液(MO)对环磷酰胺(CTX)损伤成年雄性SD大鼠生精功能的影响。方法:56只成年雄性SD大鼠(重约210~250 g)按巴戟天不同浓度,随机分为7组,空白对照组、CTX模型组、CTX+生理盐水(NS)组、CTX+10 g/kg MO组、CTX+20 g/kg MO组、CTX+30 g/kg MO组、CTX+40 g/kg MO组。以不同浓度的环磷酰胺构建大鼠生精功能损伤动物模型;巴戟天水提物用乙酸乙酯萃取后取水层成分,分别以10 g/(kg·d)、20 g/(kg·d)、30 g/(kg·d)、40 g/(kg·d)的浓度给予4组治疗组灌胃,持续2周。观察各组大鼠体重、睾丸指数、附睾指数;睾丸组织经HE染色后,观察微细结构,比较各组生精小管直径和睾丸活检评分。结果:治疗后CTX+NS组体重(234.83±28.77)g和附睾指数(2.71±0.34)与CTX+10 g/kg MO组、CTX+20 g/kg MO组、CTX+30 g/kg MO组、CTX+40 g/kg MO组之间比较无差异。CTX+NS组睾丸指数[(12.15±1.04)g]与CTX+20 g/kg MO组[(13.71±0.97)g]、CTX+30 g/kg MO组[(13.30±0.29)g]、CTX+40 g/kg MO组[(13.48±0.51)g]比较显著升高(P<0.05)。光镜下观察可见CTX+NS组明显病理改变,4个治疗组的睾丸生精小管的结构明显改善。CTX+NS组、CTX+10 g/kg MO组、CTX+20 g/kg MO组、CTX+30 g/kg MO组、CTX+40 g/kg MO组的生精小管直径分别为[(189.74±8.55、204.78±11.03、216.55±10.93、218.03±11.23、218.59±14.06)μm],睾丸活检评分分别为(5.95±1.21、9.03±0.39、9.69±0.26、9.83±0.18、9.89±0.11),CTX+NS组与4个治疗组比较存在显著性差异(P<0.05),随着巴戟天萃取液治疗浓度的提高治疗效果也随之提高。结论:巴戟天萃取液对环磷酰胺损伤的大鼠生精功能具有修复作用,并且以30 g/(kg·d)、40 g/(kg·d)的治疗浓度治疗效果最佳。
Objective: To investigate the effect of Morinda officinalis extract (MO) on spermatogenic function of adult male SD rats damaged by cyclophosphamide (CTX). Methods: Fifty-six adult male Sprague-Dawley rats weighing about 210-250 g were randomly divided into 7 groups according to the different concentrations of Morinda officinalis. The blank control group, CTX model group, CTX + NS group, CTX + 10 g / kg MO group, CTX + 20 g / kg MO group, CTX + 30 g / kg MO group and CTX + 40 g / kg MO group. The animal model of impaired spermatogenesis in rats was constructed with different concentrations of cyclophosphamide. The aqueous extract of Morinda officinalis was extracted with ethyl acetate, and the components of the aqueous layer were extracted with 10 g / (kg · d), 20 g / (kg · d ), 30 g / (kg · d), 40 g / (kg · d) were given to the four treatment groups intragastrically for 2 weeks. The body weight, testicular index and epididymis index of the rats in each group were observed. The microstructure of the testis was observed by HE staining. The diameters of the seminiferous tubules and the testicular biopsy were compared. Results: The body weight (234.83 ± 28.77) g and epididymis index (2.71 ± 0.34) in CTX + NS group were significantly higher than those in CTX + 10 g / kg MO group, CTX + , CTX + 40 g / kg MO group no difference between the comparison. Compared with CTX + 20 g / kg MO group [(13.71 ± 0.97) g] and CTX + 30 g / kg MO group [(13.30 ± 0.29) g] +40 g / kg MO group [(13.48 ± 0.51) g] was significantly higher (P <0.05). Under light microscope, the pathological changes of CTX + NS group were obvious, and the structure of testicular seminiferous tubules in 4 treatment groups was significantly improved. The diameters of seminiferous tubules of CTX + NS group, CTX + 10 g / kg MO group, CTX + 20 g / kg MO group, CTX + 30 g / ± 8.55,204.78 ± 11.03,216.55 ± 10.93,218.03 ± 11.23,218.59 ± 14.06) μm], and the scores of testicular biopsy were (5.95 ± 1.21,9.03 ± 0.39,9.69 ± 0.26,9.83 ± 0.18,9.89 ± 0.11), CTX + NS group compared with the four treatment groups there was a significant difference (P <0.05), with the increase in the concentration of Morinda officinalis extract treatment effect also increased. Conclusion: Morinda officinalis extract can repair the spermatogenic function of cyclophosphamide-injured rats, and the best therapeutic effect is achieved at the concentration of 30 g / (kg · d) and 40 g / (kg · d).