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为探讨环氧乙烷（ＥｔＯ）对ＤＮＡ分子水平的损伤作用，采用血红蛋白基因特异性的单引物，对用ＥｔＯ染毒不同时间的Ｗｉｓｔａｒ雄鼠和对照个体的肝、肾、睾丸等组织的ＤＮＡ进行半随机ＰＣＲ扩增。结果显示：在吸入染毒（８００ｍｇ／ｍ３）６～９周条件下，ＥｔＯ可引起肝、肾、睾丸等组织不同程度的ＤＮＡ损伤，其中睾丸组织ＤＮＡ损伤最为严重。
In order to investigate the effect of ethylene oxide (EtO) on DNA damage, single-primer specific for hemoglobin gene was used to detect DNA of liver, kidney and testis in Wistar rats and control individuals exposed to EtO for different time. Semi-random PCR amplification was performed. The results showed that EtO could cause different degrees of DNA damage in tissues such as liver, kidney and testis under inhalation exposure (800mg / m3) for 6 ~ 9 weeks. DNA damage in testis was the most serious.