论文部分内容阅读
AIM:To investigate the interaction between hepatitis C viruscore protein and translin protein and its role in thepathogenensis of hepatocellular carcinoma and lymphoma.METHODS:With the components of the yeast two hybridsystem 3,“bait” plasmids of HCV core the gene wasconstructed.After proving that hepatitis C virus core proteincould be firmly expressed in AH109 yeast strains,yeast two-hybrid screening was performed by mating AH109 with Y187that transformed with liver cDNA library plasmids-pACT2and then plated on quadropte dropout(QDO)medium andthen assayed for α-gal activity.Sequencing analysis of thegenes of library plasmids in yeast colonies that could growon QDO with α-gal activity was performed.The interactionbetween HCV core protein and the protein we obtained frompositive colony was further confirmed by repeating yeasttwo-hybrid analysis and coimmunoprecipitation in vitro.RESULTS:A gene from a positive colony was the gene oftranslin,a recombination hotspot binding protein.Theinteraction between HCV core protein and translin proteincould be proved not only in yeast,but also in vitro.CONCLUSION:The core protein of HCV can interact withtranslin protein.This can partly explain the molecularmechanism for hepatocellular carcinoma and lymphomacaused by HCV.
AIM: To investigate the interaction between hepatitis C viruscore protein and translin protein and its role in the pathogenensis of hepatocellular carcinoma and lymphoma. METHODS: With the components of the yeast two hybrids system 3, “bait” plasmids of HCV core the gene wasconstructed. After proving that the hepatitis C virus core protein can be expressed in AH109 yeast strain, yeast two-hybrid screening was performed by mating AH109 with Y187 transformed with liver cDNA library plasmids-pACT2 and then plated on quadropte dropout (QDO) medium and then assayed for α- gal activity. Sequencing analysis of the genes of library plasmids in yeast colonies that could growon QDO with alpha-gal activity was performed. interaction between HCV core protein and the protein we obtained from positive colony was recommed by repeating yeast two-hybrid analysis and coimmunoprecipitation in vitro .RESULTS: A gene from a positive colony was the gene of translin, a recombination hotspot binding protein interaction between HCV core protein and translin proteincould be proved not only in yeast, but also in vitro. CONCLUSION: The core protein of HCV can interact with translin protein. can not explain the molecular mechanism for hepatocellular carcinoma and lymphomacaused by HCV.