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目的:探讨Sonic Hedgehog(SHH)信号通路分子Shh和Smo在肝细胞肝癌(Hepatocellular carcinoma,HCC)中的表达及其意义。方法:用RT-PCR方法检测10例HCC组织及相应癌旁组织和肝癌细胞系HepG2、Huh7中Shh、Smo mRNA的表达。采用免疫组化方法检测30例肝癌组织及10例正常肝组织中Shh、Smo蛋白的表达。结果:RT-PCR结果显示HCC组织中Shh、Smo mRNA的表达率显著高于癌旁组织(P<0.05);Shh、Smo mRNA在Huh7中的表达强度高于HepG2,但两者间无显著性差异(P>0.05)。免疫组化结果显示Shh、Smo蛋白在HCC组织中阳性率分别为63.3%(19/30)、76.7%(23/30);Smo蛋白的表达与肿瘤大小相关(P<0.05)。Shh、Smo蛋白在正常肝组织中均无表达。结论:Shh和Smo在肝癌中高表达,表明SHH信号通路可能参与肝癌的发生,为肝癌防治提供新的依据。
Objective: To investigate the expression of Sonic Hedgehog (SHH) signaling molecules Shh and Smo in hepatocellular carcinoma (HCC) and its significance. Methods: The expression of Shh and Smo mRNA in 10 HCC tissues, corresponding paracancerous tissues and HepG2 and Huh7 cell lines were detected by RT-PCR. Immunohistochemistry was used to detect the expression of Shh and Smo proteins in 30 HCC tissues and 10 normal liver tissues. Results: The expression of Shh and Smo mRNA in HCC tissues was significantly higher than that in adjacent non-cancerous tissues (P <0.05) by RT-PCR. The expression of Shh and Smo mRNA in Huh7 was higher than that of HepG2, but there was no significant difference Difference (P> 0.05). Immunohistochemistry showed that the positive rates of Shh and Smo in HCC tissues were 63.3% (19/30) and 76.7% (23/30), respectively. The expression of Smo protein was correlated with tumor size (P <0.05). Shh, Smo protein in normal liver tissue were not expressed. Conclusion: Shh and Smo are highly expressed in hepatocellular carcinoma, indicating that SHH signaling pathway may be involved in the occurrence of hepatocellular carcinoma and provide a new basis for the prevention and treatment of liver cancer.