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通过Suzuki偶联反应,在水/DMF体系下制备了一种阴离子水溶性聚芴衍生物——聚[2,7-二溴-9,9-二(6-磷酸基己基)芴-alt-苯基]钠盐(PFHPNa).聚合物PFHPNa具有良好的水溶性和强的蓝色荧光发射.通过比较不同淬灭剂对PFHPNa和已报道的具有不同侧链长度的聚[2,7-二溴-9,9-二(6-磷酸基己基)芴-alt-苯基]钠盐(PFPPNa)的淬灭效率,研究了侧链长度对荧光传感性能的影响.对于小分子淬灭剂(甲基紫精(MV2+)和间5,10,15,20-四(N-甲基-4-吡啶基)卟吩(TMPyP4))而言,聚合物PFHPNa由于较长的侧链长度而表现出较低的灵敏度.带正电荷的金属蛋白—细胞色素c能够通过能量和电子转移将共轭聚合物的荧光淬灭.而且与PFPPNa比较,PFHPNa显示出更高的荧光淬灭.细胞色素c对聚合物PFHPNa的淬灭常数达到2.1×108L-mol-1.因此,PFHPNa可以作为细胞色素c灵敏的和选择性的荧光传感器.
An anionic water-soluble polyfluorene derivative, poly [2,7-dibromo-9,9-bis (6-phosphohexyl) fluorene-alt- Phenyl] sodium salt (PFHPNa) .Polymer PFHPNa has good water solubility and strong blue fluorescence emission.Compared the effect of different quenchers on PFHPNa and reported poly [2,7-bis Bromine-9,9-bis (6-phosphohexyl) fluorene-phenyl-phenyl] sodium salt (PFPPNa), the effect of side chain length on the fluorescence sensing performance was investigated. (MV2 +) and meta 5,10,15,20-tetrakis (N-methyl-4-pyridyl) porphine (TMPyP4)), the polymer PFHPNa, due to the longer side chain length Showed lower sensitivity.The positively charged metalloprotein, cytochrome c, was able to quench the fluorescence of the conjugated polymer through energy and electron transfer, and PFHPNa showed a higher fluorescence quench than PFPPNa.Cytochrome c to the polymer PFHPNa quenching constant of 2.1 × 108L-mol-1. Therefore, PFHPNa can be used as a cytochrome c sensitive and selective fluorescence sensor.