论文部分内容阅读
目的阐明性别与年龄对体细胞中２１号染色体分离的影响。方法用细胞松弛素Ｂ处理不同年龄及性别正常人外周血淋巴细胞获得的双核细胞，以２１号染色体近着丝粒特异性探针进行荧光原位杂交，同时检测２１号染色体丢失及不分离。结果具有４个信号点的双核细胞数与年龄的相关系数为－０．３５（Ｐ＜０．０１），２及６个信号点的双核细胞数与年龄的相关系数分别为０．１８和０．３８（Ｐ＜０．０１），２１号染色体不分离数和微核细胞数与年龄的相关系数分别为０．５６（Ｐ＜０．０１）和０．７０（Ｐ＜０．０１）。结论在不同年龄正常人外周血淋巴细胞中，微核数随年龄的增加而增加。２１号染色体不分离与年龄的关系极显著，无论在体内还是体外，２１号染色体不分离远远高于丢失。不同性别体细胞２１号染色体不分离差异不大
Objective To clarify the effect of sex and age on chromosome 21 segregation in somatic cells. Methods Binuclear cells obtained from peripheral blood lymphocytes of different ages and genders were treated with cytochalasin B. Fluorescence in situ hybridization was performed with chromosome centromere specific probe 21 and the loss and non - segregation of chromosome 21 was detected. Results The correlation coefficient between the number of binucleated cells with 4 signal points and age was -0.35 (P <0.01). The correlation coefficients between the number of binucleated cells and the age at 2 and 6 signal points were 0.18 and 0 .38 (P <0.01). The correlation coefficients between non-segregating chromosome number 21 and the number of micronucleus cells on the 21th were 0.56 (P <0.01) and 0.70 (P <0.01) respectively. Conclusion In different age normal human peripheral blood lymphocytes, micronuclei increased with age. The relationship between chromosome 21 non-segregation and age was extremely significant. The chromosome 21 was not segregated much more than the loss in vivo or in vitro. Chromosome 21 chromosomes of different sexes did not differ significantly