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目的探讨颅内动脉粥样硬化性脑梗死患者外周血CD4+CD25+FOXP3+调节性T细胞(Treg细胞)的变化及意义。方法入选50例颅内动脉粥样硬化性脑梗死患者,同时以正常人群(30例)为对照。用流式细胞分析法测定外周血中CD4+CD25+FOXP3+占CD4+细胞的比例,定量PCR检测转录因子FOXP3的mRNA水平,ELISA检测外周血血浆Treg相关细胞因子IL-10、IL-6和TGF-β的表达。结果颅内动脉粥样硬化性脑梗死患者外周血Treg/CD4+T细胞比例和FOXP3的mRNA水平显著低于正常人群组。颅内动脉粥样硬化性脑梗死患者的IL-10和TGF-β水平低于正常人群组,IL-6水平高于正常人群组。结论颅内动脉粥样硬化性脑梗死患者外周血Treg比例减少,对炎症反应的抑制作用减弱,由此可以推测Treg细胞参与了颅内动脉粥样硬化性脑梗死的发生发展。
Objective To investigate the changes and significance of CD4 + CD25 + FOXP3 + regulatory T cells (Tregs) in peripheral blood of patients with intracranial atherosclerotic cerebral infarction. Methods Fifty patients with intracranial atherosclerotic cerebral infarction were enrolled. At the same time, the normal population (30 cases) was used as control. The ratio of CD4 + CD25 + FOXP3 + CD4 + cells in peripheral blood was measured by flow cytometry. The mRNA level of FOXP3 was detected by quantitative PCR. The levels of Treg-related cytokines IL-10, IL-6 and TGF- β expression. Results The percentage of Treg / CD4 + T cells and FOXP3 mRNA in peripheral blood of patients with intracranial atherosclerotic cerebral infarction were significantly lower than those in normal controls. The levels of IL-10 and TGF-β in patients with intracranial atherosclerotic cerebral infarction were lower than those in normal people, and the levels of IL-6 were higher than those in normal people. Conclusion The decrease of Treg in the peripheral blood of patients with intracranial atherosclerotic cerebral infarction attenuates the inhibitory effect on inflammatory response. Therefore, we can infer that Treg cells are involved in the development of intracranial atherosclerotic cerebral infarction.