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目的研究宫颈正常组织、慢性炎症、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)组织、宫颈癌中树突状细胞(dendritic cells,DC)分布变化,探讨肿瘤局部免疫功能状态。方法应用免疫组化S-P法检测正常宫颈、慢性炎症、CIN组织及宫颈癌中的DC形态分布特征。结果宫颈癌组中DC高度浸润13例,低度浸润27例;CIN组中高度浸润24例,低度浸润6例;慢性炎症组中高度浸润11例,低度浸润4例;正常对照组中高度浸润10例,低度浸润5例。CIN组的DC高度浸润率高于宫颈癌组和正常对照组,相比差异有统计学意义(P<0.05),CIN组与炎症组DC高度浸润率相比差异无统计学意义(P>0.05)。宫颈癌组DC高度浸润率小于正常对照组和炎症组,差异有统计学意义(P<0.05)。结论 DC在子宫颈癌的发生、发展中起着重要的作用,反映肿瘤局部的免疫状态,为宫颈癌预后的判断和生物治疗提供实验依据。
Objective To investigate the distribution of dendritic cells (DC) in cervical normal tissue, chronic inflammation, cervical intraepithelial neoplasia (CIN) and cervical cancer, and to investigate the local immune function of tumor. Methods Immunohistochemical S-P method was used to detect the morphological distribution of DC in normal cervix, chronic inflammation, CIN and cervical cancer. Results In the cervical cancer group, 13 cases were highly infiltrated with DC and 27 cases were with low infiltration. In the CIN group, 24 cases were highly infiltrated and 6 cases were infiltrated with low infiltration. In the chronic inflammation group, 11 cases were highly infiltrated and 4 cases were infiltrated with low infiltration. High infiltration in 10 cases, 5 cases of low infiltration. The positive rate of DC in CIN group was significantly higher than that in cervical cancer group and normal control group (P <0.05), but there was no significant difference between CIN group and inflammatory group (P> 0.05) ). Cervical cancer group DC invasive rate was less than the normal control group and inflammation group, the difference was statistically significant (P <0.05). Conclusions DC plays an important role in the occurrence and development of cervical cancer, reflecting the local immune status of the tumor, providing an experimental basis for judging the prognosis of cervical cancer and biotherapy.