Vogt-小柳-原田病患者对黑素细胞特异性蛋白KU-MEL-1的频发免疫反应

• 作 者: Otani S. Sakurai T. Yamamoto K. Y. Kawakami 邢咏新
• 机 构: Division of Cellular Signaling Institute for Advanced Medical Research Keio University School of Me
• 出 处: 世界核心医学期刊文摘.眼科学分册
• 发表日期: 2006年12期
• 关键词: 黑素细胞 原田病 小柳 免疫反应 葡萄膜炎 抗体 特异性蛋白 黑色素瘤细胞系 IgG λ噬菌体

Aim: To isolate autoantigens possibly involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: Autoantigens recognised by immunoglobulinGantibodies (IgG Ab) in sera from VKH patients were isolated by screening the lambda phage cDNA libraries made from melanocytes and a highly pigmented melanoma cell line with the patients’sera. Presence of IgG specific for the autoantigens in sera from patients with various panuveitis and healthy individuals was evaluated. Relation between the specific IgG and various clinicopathological features was examined. Results: KU-MEL-1 was found to be one of the 81 isolated positive clones representing 35 distinct genes, which is a previously isolated melanoma antigen preferentially expressed in melanocytes. The IgG Ab specific for KU-MEL-1 was detected in sera from patients with VKH in significantly higher amounts than in sera from patients with Behcet’s disease, sarcoidosis, and from healthy individuals. Positive serum KU-MEL-1 Ab was significantly associated with HLA-DRB10405 and male VKH patients. Conclusion: KU-MEL-1 was identified as a new autoantigen for VKH. The highly frequent induction of IgG Ab for KU-MEL-1 in HLADRB10405 positive VKH patients may suggest the possible involvement of KU-MEL-1 specific CD4+T cells in the pathogenesis of VKH, suggesting the possible use in the development of diagnostic and therapeutic treatments for VKH patients.

Aim: To isolate autoantigens possibly involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: Autoantigens recognized by immunoglobulin Antibodies (sepharose) in sera from VKH patients were isolated by screening the lambda phage cDNA libraries made from melanocytes and a highly pigmented melanoma cell line with the patients’ serum. Presence of IgG specific for the autoantigens in sera from patients with various panuveitis and healthy individuals was evaluated. Results: KU-MEL-1 was found to be one of the 81 isolated positive clones representing 35 distinct genes, which is previously isolated from melanoma antigen preferentially expressed in melanocytes. The IgG Ab specific for KU-MEL-1 was detected in sera from patients with VKH in significantly higher amounts than in sera from patients with Behcet’s disease, sarcoidosis, and from healthy individuals. Positive serum KU-MEL-1 Ab was Significant associated with HLA-DRB1 * 0405 and male VKH patients. Conclusion: KU-MEL-1 was identified as a new autoantigen for VKH. The highly frequent induction of IgG Ab for KU-MEL-1 in HLADRB1 * 0405 positive VKH patients may suggests the possible involvement of KU-MEL-1 specific CD4 + T cells in the pathogenesis of VKH, suggesting the possible use in the development of diagnostic and therapeutic treatments for VKH patients.