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选用两种抗旱性不同的冬小麦——太原633(抗旱)和C609(干旱敏感),研究了水分胁迫条件下植物蛋白质代谢变化的规律及与植物抗旱性的关系.双向电泳结果表明,2.0MPa PEG-6000水分胁迫引起了两品种冬小麦叶片蛋白质变化的明显差异.抗旱品种干旱诱导蛋白及其他蛋白变化早于干旱敏感品种,初步证实了抗旱性不同的品种在水分胁迫时蛋白质代谢变化的时间顺序.干旱诱导的蛋白质的变化包括干旱诱导蛋白及蛋白质含量的变化在复水后均可迅速恢复;蛋白质转录抑制剂——放线菌素D可抑制干旱诱导蛋白的产生,说明其基因表达主要在转录水平进行调控.
Two wheat cultivars with different drought resistance (drought-tolerant) and C609 (drought-sensitive) were selected to study the relationship between the changes of plant protein metabolism and plant drought resistance under water stress conditions.The results of two-dimensional electrophoresis showed that 2.0MPa PEG -6000 water stress caused significant differences in the protein changes of winter wheat leaves of two varieties.Drought-induced drought-induced proteins and other proteins in drought-resistant varieties were earlier than those in drought-sensitive cultivars, and the temporal sequence of protein metabolism in different drought-tolerant cultivars was initially confirmed. Drought-induced changes in proteins, including drought-induced protein and protein content, recovered quickly after rehydration; protein transcription inhibitor-dactinomycin D inhibited the production of drought-inducible protein, indicating that its gene expression mainly transcribed Level regulation.