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目的:探讨小鼠胸腺内注射异基因抗原在同种异体异基因坐骨神经移植免疫耐受中的作用。方法:自供体小鼠C57BL/6的脾细胞中提取MHC抗原注入受体鼠Balb/c小鼠胸腺内,于2后周移植供体鼠坐骨神经。48只Balb/c小鼠随机分为4组,A组(胸腺内注射组)、B组(自体神经移植组)、C组(冷冻异体神经移植组)、D组(异体神经移植加用免疫抑制剂组)。于3周后进行电生理学、组织学、免疫学检测。结果:运动神经传导速度,A组(38.23m/s)与D组(36.39I彬S)相比无显著性差异(p>0.05),组织学、电镜、免疫学(混合淋巴细胞培养及迟发性超敏反应)检测结果均证实B组分别优于A组、D组、C组。结论:胸腺内注射异基因MHC抗原可诱导对异体坐骨神经移植的特异性免疫耐受。
Objective: To investigate the effect of intrathymus injection of allogeneic antigen on immune tolerance of allogeneic sciatic nerve graft. METHODS: MHC antigens were extracted from the spleen cells of donor C57BL / 6 mice and injected into the thymus of Balb / c mice. The sciatic nerve was transplanted to the donor mice at 2 weeks after transplantation. Forty-eight Balb / c mice were randomly divided into four groups: group A (thymus injection group), group B (autograft group), group C (cryo-allograft nerve transplantation group), group D Inhibitor group). After 3 weeks electrophysiology, histology, immunological testing. Results: There was no significant difference in motor nerve conduction velocity between group A (38.23m / s) and group D (36.39%), histological, electron microscopy, immunology Hair hypersensitivity) test results confirmed that B group were better than A group, D group, C group. Conclusion: Intrathymic injection of allogeneic MHC antigen can induce specific immune tolerance to allogeneic sciatic nerve transplantation.