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目的研究扇贝裙边糖胺聚糖(glycosaminoglycan from Scallop Skirt,SS-GAG)体外抗单纯疱疹病毒Ⅰ型(HSV-Ⅰ)的作用。方法运用单纯疱疹病毒Ⅰ型(HSV-ⅠSM44株),并以非洲绿猴肾细胞(Vero细胞)为宿主细胞,通过观察病毒感染后的细胞变性反应(cytopathic effect,CPE)和运用MTT比色法,检测不同浓度药物SS-GAG对Ⅰ型单纯疱疹病毒是否有直接的灭活作用、对HSV-Ⅰ感染复制的抑制活性以及对HSV-Ⅰ感染细胞的综合作用等,并观察药物对Vero细胞的毒性作用。结果与病毒对照组相比,SS-GAG各浓度组(100、50、25mg.L-1)能有效地保护经HSV-Ⅰ感染的Vero细胞,使细胞活性增强(P<0.01);并减弱HSV-Ⅰ导致的病变效应,抑制病毒的复制。此作用随着药物浓度的增加而增强。但是SS-GAG对HSV-Ⅰ没有直接的灭活作用;SS-GAG在50~1600mg.L-1浓度范围内对Vero细胞无明显的细胞毒性。结论SS-GAG在体外实验系统中显示出明显的保护宿主细胞抵抗HSV-Ⅰ病毒感染的活性作用。
Objective To study the anti-herpes simplex virus type I (HSV-I) effect of scallop skirt glycosaminoglycan from Scallop Skirt (SS-GAG) in vitro. Methods Herpes simplex virus type 1 (HSV-ISM44 strain) and Vero cells were used as host cells to observe cytopathic effect (CPE) after viral infection and MTT colorimetric method was used. ,To test whether different concentrations of drug SS-GAG can directly inactivate the type I herpes simplex virus, inhibit the replication of HSV-I infection and synthesize the HSV-I infected cells, and observe the effects of drugs on Vero cells. Toxic effect. Results Compared with the virus control group, SS-GAG concentrations (100, 50, 25 mg.L-1) were effective in protecting HSV-I-infected Vero cells and increased cell viability (P<0.01); HSV-I causes lesion effects and inhibits viral replication. This effect increases with increasing drug concentration. However, SS-GAG had no direct inactivation effect on HSV-I; SS-GAG had no obvious cytotoxicity to Vero cells in the range of 50-1600 mg.L-1. Conclusion SS-GAG showed obvious protective activity against HSV-I virus infection in vitro.