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利用分葱茎尖培养成功地诱导了体细胞胚胎发生。具体步骤如下：Ⅰ．在ＭＳ＋２，４－Ｄ１ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上诱导愈伤组织；Ⅱ．在ＭＳ＋２，４－Ｄ０．５ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上进行愈伤组织的继代培养，继代培养３次以后，转变为胚性愈伤组织；Ⅲ．在ＭＳ＋２，４－Ｄ０．２５ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上进行胚状体的诱导；Ⅳ．在无任何激素的ＭＳ培养基上使胚状体再生植株。切片观察表明，胚状体大多数起源于愈伤组织表层及其以下细胞。
Somatic embryogenesis was successfully induced by subling shoot tip culture. Specific steps are as follows: Ⅰ. The callus was induced on medium MS + 2,4-D1mg / L + BA2.0mg / L; Ⅱ. The callus was subcultured on MS + 2,4-D0.5mg / L + BA2.0mg / L medium and subcultured into embryogenic callus after subculture three times; Ⅲ. Induction of embryoid bodies on MS + 2,4-D0.25mg / L + BA2.0mg / L medium; Ⅳ. Embryoid bodies are regenerated on MS medium without any hormones. Slice observation showed that the majority of embryoid bodies originated from the callus surface and below cells.