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目的:应用显微拉曼光谱技术探讨人体死亡后脾组织细胞DNA含量变化与死亡时间间隔(PMI)的变化规律,为推断死亡时间提供检测依据。方法:选取8例已知死亡时间的人体脾脏置于特定环境下,在死后48 h~72 h范围内不同时间点提取人脾组织样品,利用激光共焦显微拉曼光谱技术,在拉曼位移测试范围800 cm-1~3200 cm-1内检测样品的化学基团的构建及含量变化。结果:人死后48 h~72 h内,随着PMI的推移,脾组织显微拉曼光谱的特征峰峰位没有发生明显变化,但特征峰峰高有明显变化;与核酸有关的峰位1094 cm-1处峰强度随PMI有明显下降趋势;与脂类有关的峰位1454 cm-1、2923 cm-1处的峰强随PMI变化不明显;每条谱线的相对峰强(I1094/I2923)随死亡时间的推移而减小。结论:人死亡后脾组织细胞DNA含量与PMI有明显的相关性。
OBJECTIVE: To investigate the changes of DNA content and the time interval of death (PMI) in human spleen cells after the death of human beings by using Raman microscopy, and to provide the basis for inferring the time of death. Methods: Eight human spleens with a known death time were selected and placed in a specific environment. Human spleen samples were collected at different time points from 48 h to 72 h after death. The laser scanning confocal microscopy (Raman) Construction and content changes of chemical groups in the test range of 800 cm-1 to 3200 cm-1. RESULTS: Within 48 hours to 72 hours after death, the characteristic peak position of microscopic Raman spectrum of spleen tissue did not change significantly with the PMI shift, but the peak height of characteristic peak obviously changed. The peak position related to nucleic acid The peak intensity at 1094 cm-1 decreased significantly with PMI; the peak intensity at 1454 cm-1 and 2923 cm-1 at lipid peak was not obvious with PMI; the relative peak intensity of each line (I1094 / I2923) decreases with the passage of time. Conclusion: There is a significant correlation between the DNA content of spleen cells and PMI after human death.