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目的:比较线粒体基因组(mitochondrialDNA,mtDNA)拷贝数在胃癌和癌旁正常组织间的差异,探究mtDNA与胃癌发生的关系。方法:PCR分别扩增胃癌组织和癌旁正常粘膜组织各20例共40个样本的线粒体D-loop区两个高变区HV1(Hypervariableregion)和HV2;并以核基因组的β-actin作为定量标准物。聚丙烯酰胺凝胶电泳(Polyacrylamidegelelectrophoresis,PAGE)银染比较mtDNA拷贝数在癌和正常组织间的差异。结果:HV1和HV2拷贝量(用β-actin标准化)在胃癌组织和胃正常组织间有显著的差异,P<0.01;其拷贝量与环腺苷酸磷酸二酯酶(cAMP-PDE)和环鸟苷酸磷酸二酯酶(cGMP-PDE)表达有统计学联系,P<0.05。结论:胃癌发生与mtDNA拷贝量的减少有着密切的关系,可能成为一种新的肿瘤分子标志物。
OBJECTIVE: To compare the differences in copy numbers of mitochondrial DNA (mtDNA) between normal gastric mucosa and normal gastric mucosa and to explore the relationship between mtDNA and gastric carcinogenesis. Methods: Hypervariable region HV1 (HV1) and HV2 in 20 mitochondrial D-loop regions of 20 specimens of gastric cancer tissue and adjacent normal mucosa were respectively amplified by PCR. Β-actin of nuclear genome was used as a quantitative standard Things. Polyacrylamide gel electrophoresis (PAGE) silver staining compared mtDNA copy number in cancer and normal tissue differences. RESULTS: There was a significant difference in HV1 and HV2 copy number (normalized by β-actin) between gastric cancer tissue and normal gastric tissue, P <0.01. The copy number of HV1 and HV2 was significantly correlated with cAMP-PDE and The expression of guanylate phosphodiesterase (cGMP-PDE) was statistically significant (P <0.05). Conclusion: There is a close relationship between the occurrence of gastric cancer and the decrease of mtDNA copy number, which may become a new tumor molecular marker.