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为研究鼻咽癌基因治疗的可能性，应用ＥＢ病毒反义ＬＭＰ１基因的重组逆转录病毒载体，用ＰＡ３１７细胞包装成假型逆转录病毒，免疫荧光检查该病毒能明显抑制Ｂ９５－８细胞内ＥＢ病毒的激活，抑制率达７１％。用此病毒成功地感染了人低分化鼻咽癌ＣＮＥ－３细胞株，使ＣＮＥ－３细胞生长速率下降约７０％，软琼脂集落形成和裸鼠致瘤能力均明显下降。用Ｓｏｕｔｈｅｒｎ杂交证实转染的肿瘤组织细胞中有较高水平的ｐＺＩＰ载体ｎｅｏ基因存在，证实了ＥＢ病毒反义ＬＭＰ基因能有效地抑制人低分化鼻咽癌ＣＮＥ－３细胞的生长和裸鼠致瘤能力，为鼻咽癌的基因治疗提供了实验依据。
In order to study the possibility of NPC gene therapy, recombinant retroviral vector of Epstein-Barr virus antisense LMP1 gene was used and packaged as pseudotyped retrovirus with PA317 cells. Immunofluorescence showed that the recombinant retroviral vector could significantly inhibit the expression of EB in B95-8 cells Virus activation, the inhibition rate of 71%. With this virus successfully infected human poorly differentiated nasopharyngeal carcinoma CNE-3 cell lines, so that CNE-3 cell growth rate decreased by about 70%, soft agar colony formation and nude mice tumor formation were significantly decreased. Southern blotting confirmed that there was a high level of pZIP vector neo gene in the transfected tumor cells, which confirmed that Epstein-Barr virus antisense LMP gene could effectively inhibit the growth of human poorly differentiated NPC CNE-3 cells and the growth of nude mice Tumor capacity, for the gene therapy of nasopharyngeal carcinoma provided an experimental basis.