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目的采用HPLC法测定前列康泰胶囊中的苦参碱与氧化苦参碱。方法采用Kromasil NH2色谱柱(150mm×4.6mm,5μm),流动相为乙腈-无水乙醇-3%磷酸(80:10:10),流速1.0mL·min-1,检测波长220nm。结果苦参碱和氧化苦参碱的线性范围分别为0.390～1.562、0.258～1.030μg;r分别为0.9998、0.9996;平均回收率分别为97.1%(RSD=0.36%,n=6)、97.5%(RSD=0.54%,n=6)。结论所用方法灵敏、准确、专属性强,可用于前列康泰胶囊的质量控制。
OBJECTIVE To determine matrine and oxymatrine in Qianliekangtai capsule by HPLC. Methods Kromasil NH2 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-absolute ethanol-3% phosphoric acid (80:10:10). The flow rate was 1.0 mL · min-1. Results The linear ranges of matrine and oxymatrine were 0.390-1.562 and 0.288-1.030μg, respectively. The average recoveries were 97.1% (RSD = 0.36%, n = 6), 97.5% (RSD = 0.54%, n = 6). Conclusion The method used is sensitive, accurate and specific and can be used for the quality control of Qianliekangtai capsule.