论文部分内容阅读
以盾叶薯蓣的块茎、茎、叶和茎尖为材料，对其进行了愈伤组织培养，并根据愈伤组织个体间生长速度和皂甙含量差异，进行了高产系的筛选．结果表明：盾叶薯蓣不同外植体（块茎、茎、叶、茎尖）均能诱导出愈伤组织，其中以茎尖形成愈伤组织最快，皂甙元含量最高；不同培养基、ｐＨ 值、接种量、温度、激素等因子对愈伤组织的形成、生长及皂甙元含量有很大的影响；改良 Ｍ Ｓ培养基、２，４－Ｄ 浓度为２．０～４．０ ｍ ｇ／Ｌ、ＢＡ 为 ０．５ ｍ ｇ／Ｌ 的激素配比使愈伤组织的生长量、产物含量最高；对无性系进行筛选和 ＨＰＬＣ 检测，以 １２号无性系生长速率和皂甙元含量最高，其皂甙含量达 ３．７％ ，生长量比普通系高２１．５％ ．
The tuber, stem, leaf and stem apex of Dioscorea zingiberensis as material were used for callus culture. The high-yielding lines were screened according to the difference of growth rate and saponin content between callus individuals. The results showed that callus could be induced from different explants (tubers, stems, leaves and shoot tips) of Dioscorea zingiberensis. The callus formation was the fastest and the content of saponin was the highest. , Inoculum size, temperature, hormone and other factors on callus formation, growth and sapogenin content have a great impact; modified M S medium, 2,4-D concentration of 2.0 ~ 4.0 m g / L and BA 0.5 m g / L, the callus growth and product content were the highest. Clonal screening and HPLC detection showed that clonal growth rate and sapogenin content were the highest in clones Saponin content of 3.7%, the growth of 21.5% higher than normal department.