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目的 :初步探讨外周血NK细胞和B细胞在严重急性呼吸综合征 (severeacuterespiratorysyndrome ,SARS)发病进程及发病机理中的意义。方法 :应用流式细胞仪检测和分析SARS病人外周血B淋巴细胞、NK细胞动态变化 ,使用的NK细胞表面标志为CD16 + CD5 6、B细胞表面标志为CD19。结果分析用BD公司的MultiSet自动分析软件。结果 :随着病程的进展92 %的SARS病人B细胞先降后升或持续升高 ,少部分病人B细胞无改变或下降。NK细胞显示不同的个体差异 ,73.7%的病人显示典型的抗病毒免疫现象 ,即发病初期NK细胞数升高 ,恢复期NK细胞数下降 ,也有部分病人NK细胞无变化 (15 .8% )或升高 (10 5 % )。结论 :在SARS发病进程中B淋巴细胞介导的体液免疫可能起一定的作用。
Objective: To investigate the significance of peripheral blood NK cells and B cells in the pathogenesis and pathogenesis of severe acute respiratory syndrome (SARS). Methods: Flow cytometry was used to detect and analyze the dynamic changes of peripheral blood B lymphocytes and NK cells in patients with SARS. The surface markers of NK cells were CD16 + CD5 6 and the surface markers of B cells were CD19. Results analysis using BD’s MultiSet automatic analysis software. Results: With the progression of disease, B cells in 92% of SARS patients first and then decreased or continued to increase, and in a minority of patients, B cells did not change or decline. NK cells showed different individual differences, 73.7% of the patients showed typical anti-virus immune phenomenon, that is, the number of NK cells in the early stage of the disease increased, the number of NK cells in the recovery period decreased, and some patients had no change in NK cells (15.8%) or Increase (105%). Conclusion: B lymphocyte-mediated humoral immunity may play a role in the pathogenesis of SARS.