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目的:观察小肠黏膜下层(small intestinal submucosa,SIS)和脱细胞心包(pericardium,PC)修复大鼠腹壁缺损的效果,比较两种生物材料相容性。方法:SD大鼠40只,体重200～250g,手术造成3 cm×2 cm全层腹壁缺损,随机分为二组(n=20),分别采用相同面积的小肠黏膜下层(small intestinal submucosa,SIS)和脱细胞真皮基质(acellular dermal matr,ADM)补片进行修补。术后1、2、4和8周分批取出腹壁修复材料,行动物一般情况观察、腹腔内粘连情况评价、力学强度测定及组织学观察。结果:术后动物都成活,两种材料术后8周均无疝瘘发生,缺损得到完整修复。术后各期SIS组的腹腔粘连评分明显低于PC组。术后4、8周,SIS组力学强度强于PC组,有统计学意义;组织学观察两组未见明显免疫排斥反应,SIS组的组织再生和重塑、血管化优于PC组;术后炎症反应两组无明显差异。结论:SIS和PC均能修复大鼠腹壁全层缺损,SIS在生物相容性方面优于PC。
Objective: To observe the effects of small intestinal submucosa (SIS) and pericardium (PC) on the repair of abdominal wall defects in rats and to compare the compatibility of the two biomaterials. Methods: Forty SD rats weighing 200-250g were randomly divided into two groups (n = 20). Small intestinal submucosa (SIS) of the same area ) And acellular dermal matr (ADM) patch for repair. Abdominal wall repair materials were removed in batches at 1, 2, 4, and 8 weeks after operation. The general observation of animals, the evaluation of intra-abdominal adhesions, the determination of mechanical strength and histological observation were performed. Results: All the animals survived. There were no hernia fistulas in both groups after 8 weeks of operation, and the defects were completely repaired. The score of intraperitoneal adhesion in SIS group after operation was significantly lower than that in PC group. At 4 and 8 weeks after operation, the mechanical strength of SIS group was stronger than that of PC group, and there was no significant difference between the two groups (P> 0.05). There was no obvious immunological rejection in the two groups. The tissue regeneration and remodeling of SIS group was better than that of PC group Post-inflammatory reaction was no significant difference between the two groups. CONCLUSIONS: SIS and PC can both repair the full-thickness defect of the abdominal wall in rats. SIS is superior to PC in biocompatibility.