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以7a生曙光油桃为试材,通过冰冻低温处理枝条,研究了不同温度和时间对桃芽的存活率、萌芽级数和活性氧含量及其相关酶活性的影响,并探讨对桃芽自然休眠的调控效应。结果表明,短时间冰冻低温处理对桃芽自然休眠解除的调控效应因处理时期、处理温度和处理持续时间长短的不同而异。3个不同采样时期,短时间冰冻处理中,-4℃和-7℃处理对油桃芽休眠解除的效应不明显,桃芽中H2O2(过氧化氢)含量、O·2(超氧阴离子自由基)产生速率和·OH(羟基自由基)产生速率及POD(过氧化物酶)、CAT(过氧化氢酶)和SOD(超氧化物歧化酶)活性与对照相比差异不大;但-10℃、-13℃、-16℃、-19℃和-21℃各处理对油桃芽休眠解除呈正调控效应,桃芽内除SOD活性显著降低外其它指标都明显升高;但是采样时期越晚,调控效应越明显。H2O2含量、O·2和·OH产生速率的显著增加表明短时间冰冻处理可有效解除芽自然休眠,并且活性氧的迅速增加可能是短时间冰冻处理解除芽自然休眠的重要因素。
The effects of different temperature and time on the survival rate, germination stages and reactive oxygen species content and the activity of related enzymes of peach buds were studied by using 7 dawn dawn nectarines as experimental materials. Hypnosis regulation effect. The results showed that the control effect of short-term frozen low-temperature treatment on the natural dormancy release of peach buds varied with the duration of treatment, the treatment temperature and the duration of treatment. In 3 different sampling periods, the effect of freezing at -4 ℃ and -7 ℃ on dormancy of nectarine buds was not obvious in short-time freezing treatment. The content of H2O2 (hydrogen peroxide) in peach buds, O2 · (superoxide anion free radical (OH radical) production rate and POD (peroxidase), CAT (catalase) and SOD (superoxide dismutase) activity were not significantly different from the control; but - The treatments of 10 ℃, -13 ℃, -16 ℃, -19 ℃ and -21 ℃ all showed a positive regulation effect on the dormancy release of nectarine buds, and the other indexes were significantly increased except the SOD activity in the peach buds. However, Late, the more obvious regulatory effects. The significant increase of H2O2 content, O · 2 and · OH production rates indicated that short-term freezing treatment could effectively relieve the natural dormancy of buds, and the rapid increase of reactive oxygen species may be an important factor in the natural dormancy release by short-term freezing treatment.