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目的探讨S100钙结合蛋白A4(S100 calcium-binding protein A4,S100A4)和骨桥蛋白(osteopontin,OPN)在大鼠糖尿病视网膜病变(diabetic retinopathy,DR)中的表达及意义。方法选取2015年3—11月健康雄性SD大鼠18只,用抛硬币法随机选取9只进入糖尿病组,另9只进入对照组。一次性腹腔注射链脲佐菌素(streptozocin,STZ)65 mg/kg建立糖尿病大鼠模型。注射3 d后尾静脉采血测血糖浓度,连续三次>250 mg/dl为造模成功。4周后腹腔注射水合氯醛麻醉,处死。解剖取视网膜,速冻,-70℃保存。用同样方法取对照组大鼠视网膜。用ELISA法检测S100A4和OPN的浓度。计量资料采用t检验,P<0.05为差异有统计学意义。结果糖尿病组大鼠视网膜S100A4和OPN[(24.80±1.01)、(206.37±11.78)ng/L]与对照组比较[(11.42±1.37)、(99.94±9.23)ng/L],浓度均升高,对比差异均有统计学意义(均P<0.05)。结论 S100A4和OPN是DR新的生物标志物,S100A4和OPN表达下调可能会有效抑制DR的进展。
Objective To investigate the expression and significance of S100 calcium-binding protein A4 (S100A4) and osteopontin (OPN) in diabetic retinopathy (DR) in rats. Methods Eighteen healthy male Sprague-Dawley rats from March to November in 2015 were selected. Nine rats were randomly selected into the diabetic group by the coin-tossing method, and the other nine rats entered the control group. A single intraperitoneal injection of streptozocin (streptozocin, STZ) 65 mg / kg diabetic rat model. After 3 days of injection, the tail vein blood was collected to measure the blood glucose level. Three times in succession> 250 mg / dl was successful in modeling. Four weeks after intraperitoneal injection of chloral hydrate anesthesia, sacrificed. Anatomy retina, frozen, -70 ℃ preservation. Using the same method to take control rat retinas. The concentrations of S100A4 and OPN were measured by ELISA. Measurement data using t test, P <0.05 for the difference was statistically significant. Results Compared with the control group, the levels of S100A4 and OPN in retina of diabetic group were significantly higher than those of control group [(11.42 ± 1.37), (99.94 ± 9.23) ng / L] ([24.40 ± 1.01], (206.37 ± 11.78) ng / L] , The differences were statistically significant (P <0.05). Conclusion S100A4 and OPN are new biomarkers of DR. Down-regulation of S100A4 and OPN may effectively inhibit the progression of DR.