论文部分内容阅读
目的观察大鼠C6胶质瘤模型放疗前后氢质子磁共振波谱(1H-MRS)表现,探讨其在早期监测肿瘤治疗反应中的作用。方法建立SD大鼠C6胶质瘤模型,分为照射组(n=10)和未照射组(n=12),建模后第9天给予照射组荷瘤鼠单次大剂量外照射(15 Gy/次/野)。两组大鼠建模后均接受1H-MRS扫描,选择N-乙酰天冬氨酸(NAA)、胆碱(Cho)、乳酸(Lac)为检测肿瘤组织代谢的指标,同时行磁共振扫描(MRI)检测肿瘤体积的变化;连续观察建模后两组代谢物及体积变化,并对两组代谢指标变化与肿瘤体积变化行相关性分析。结果所有大鼠均成功种植肿瘤;未照射组随肿瘤体积增大,Cho、Lac浓度增加,NAA浓度减小,代谢物变化与体积变化呈显著相关性(Cho:r=0.958,P<0.001;Lac:r=0.989,P<0.001;NAA:r=-0.943,P<0.001);照射组放疗后1 d,Cho由3.37 mmol/kg降至2.0 mmol/kg(P<0.01)。放疗后3 d,Lac由2.34 mmol/kg降至1.59 mmol/kg(P<0.01),NAA较治疗前无显著改变(P>0.05),放疗后3 d肿瘤体积较治疗前增加31.4%(P<0.01),放疗后5 d体积开始减小,随后出现肿瘤生长抑制,各代谢指标继续下降且与体积变化呈相关趋势(Cho:r=0.696,P=0.304;Lac:r=0.911,P=0.089;NAA:r=-0.583,P=0.417),MRI检测的体积发生变化之前,1H-MRS检测的代谢物已经出现抑制性变化。结论1H-MRS可无创性获得胶质瘤放疗前后代谢物NAA、Cho、Lac变化信息,较早监测早期治疗反应,敏感性优于MRI,是一种有效的判断近期疗效的检查方法。
Objective To observe the expression of proton magnetic resonance spectroscopy (1H-MRS) before and after radiotherapy in rat C6 glioma model and to explore its role in early monitoring of tumor response. Methods The C6 glioma model was established in SD rats. The rats were divided into irradiation group (n = 10) and non-irradiation group (n = 12) Gy / times / wild). The rats in both groups were subjected to 1H-MRS scans after modeling, and NAA, Cho and Lac were chosen as the indicators of tumor tissue metabolism. At the same time, magnetic resonance MRI) were used to detect the change of tumor volume. The metabolites and volume changes of the two groups were observed continuously after modeling. Correlation analysis was made between the change of metabolic index and tumor volume. Results The tumors were successfully grown in all the rats. With the increase of the tumor volume, the concentration of Cho and Lac increased and the concentration of NAA decreased in the non - irradiation group. The metabolites showed significant correlation with the volume change (Cho: r = 0.958, P <0.001) Cho: 0.989, P <0.001; NAA: r = -0.943, P <0.001). The Cho decreased from 3.37 mmol / kg to 2.0 mmol / kg on the 1st day after radiotherapy (P <0.01). At 3 days after radiotherapy, Lac decreased from 2.34 mmol / kg to 1.59 mmol / kg (P <0.01), NAA had no significant change (P> 0.05), and the tumor volume increased 31.4% (P <0.01). After 5 days of radiotherapy, the volume began to decrease, and then the tumor growth was inhibited. The metabolic indexes continued to decrease and correlated with the volume changes (Cho: r = 0.696, P = 0.304; 0.089; NAA: r = -0.583, P = 0.417). The metabolites detected by 1H-MRS have shown inhibitory changes before the volume of MRI examination changes. Conclusion 1H-MRS can be noninvasively obtained before and after radiotherapy of glioma metabolites NAA, Cho, Lac changes in the early monitoring of early response to treatment, the sensitivity is superior to MRI, is an effective method to determine the short-term efficacy of the test.