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应用 Maximow 双盖片法培养新生大白鼠颈上节植块。一部分培养物的培养液添加神经生长因子(NGF)粗制剂,另一部分未用 NGF,两者均获得生长。但应用 NGF 的培养物比不用 NGF 的培养物生长快速。NGF 对神经突起的生长和维持神经元的生存有极显著的促进作用。神经突起可单条或集合成束呈辐射状生长,或交织成网,或象树枝样向四周扩展。神经突起末端的生长锥是生长最活跃的部分。雪旺细胞呈梭形或线形,与成纤维细胞容易鉴别,它们常贴附神经突起生长。本研究并藉相差显微缩时电影摄影,记录了神经突起及其末端生长锥的生长活动状况和雪旺细胞的有丝分裂过程。交感神经元的胞体停留在植块内,未见外移。
Application of Maximow double-cover method to culture neonatal rats with cervical spine. A portion of the culture broth was supplemented with a crude formulation of nerve growth factor (NGF) and the other without NGF, both of which were grown. However, cultures using NGF grew faster than cultures without NGF. NGF plays a very significant role in promoting neurite outgrowth and maintaining neuronal survival. Neurites can be single or set into a bundle of radial growth, or interwoven into a network, or like a tree-like expansion to the surrounding. The growth cone at the tip of the neurite is the most actively growing part. Schwann cells are fusiform or linear, and fibroblasts easily identified, they often attached to neurite growth. In this study, phase contrast microscopic time-lapse photography was used to record the growth activities of neurites and their terminal cones and the mitotic process of Schwann cells. Sympathetic neurons remain in the plant cell body, no external migration.