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Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution are also very important for Ca to accomplish its function at the whole-plant level. In this experiment, one-year-old apple seedlings (M. hupehensis Rehd.) were investigated to determine the distribution of stored Ca, the different forms of Ca, and Ca2+-ATPase activity after treatment with indole butyric acid (IBA). The results showed that the total Ca measured in mature leaves and Ca2+- ATPase activity in tender leaves were higher compared with those in the control (CK). Calcium nitrate and calcium chloride (ALc-Ca) and calcium phosphate and calcium carbonate (HAC-Ca) decreased in both mature leaves and shoots, whereas water-soluble calcium (H2O-Ca), calcium pectate (NaCl-Ca), and calcium oxalate (HCl-Ca) increased. The percentage of active calcium, calcium pectate, and water-soluble calcium increased, whereas the percentage of calcium phosphate and calcium carbonate decreased. When treated with IBA, calcium fractions and percentage of the different forms of Ca was enhanced in 40 part per million (ppm) IBA compared with 20 ppm IBA and water. The results indicated that IBA increased the percentage of both active calcium (NaCl-Ca and H2O-Ca) in tender shoots and boosted the transportation of stored Ca in plants. IBA promoted Ca2+-ATPase activity and Ca2+ uptake in tender shoots of M. hupehensis. It can improve the total Ca contents and the relative percentage of Ca.
Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca2 + in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution are also very important for Ca to accomplish its function at the whole-plant level. In this experiment, one-year-old apple seedlings (M. hupehensis Rehd.) were investigated to determine the distribution of stored Ca, the different forms of Ca, and Ca2 + -ATPase activity after treatment with indole butyric acid (IBA). The results showed that the total Ca measured in mature leaves and Ca2 + - ATPase activity in tender leaves were higher than those in the control (CK). Calcium nitrate and calcium chloride (ALc-Ca) and calcium phosphate and Calcium carbonate (HAC-Ca) decreased in both mature leaves and shoots, whereas water-soluble calcium (H 2 -Ca), calcium pectate (NaCl-Ca), and calcium oxalate (HCl-Ca) increased. calcium pectate, and water-s When treated with IBA, calcium fractions and percentage of the different forms of Ca was enhanced in 40 part per million (ppm) IBA compared with 20 ppm IBA and water. The IBA promoted Ca2 + -ATPase activity and Ca2 + uptake in tender shoots of M. hupehensis. IBA promoted Ca2 + -ATPase activity and Ca2 + uptake in tender shoots of M. hupehensis. IBA promoted the percentage of both active calcium (NaCl-Ca and H2O-Ca) in tender shoots and boosted the transportation of Ca. It can improve the total Ca contents and the relative percentage of Ca.