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研究了一种拟用于SPR生物传感器的生物材料。为此,首先在金膜表面制备聚丙烯酰吡咯(PAP)膜,然后对膜的结构和表面形貌进行了研究。其中,制备PAP的方法是以丙烯酰氯和吡咯钾盐合成丙烯酰吡咯,然后以偶氮二异丁腈(AIBN)为引发剂,进行自由基聚合;金膜表面制备PAP膜的方法是:PAP溶于N,N-二甲基甲酰胺(DMF),然后旋涂到金膜上;最后,涂膜表面与吡咯在三氯化铁溶液中化学法聚合,形成聚合吡咯层。红外光谱可以看出,所得聚合物就是需要的产品;AFM非原位表面形貌图像和三维立体形貌图上显示,涂膜覆盖了金膜表面原有的小孔,表面粗糙度比纯金膜增大。
A biomaterial intended for SPR biosensors was investigated. To this end, the polyacrylamide (PAP) film was first prepared on the gold film surface, and then the structure and surface morphology of the film were studied. Among them, the preparation of PAP by acryloyl chloride and pyrrole potassium synthesis of acryloylpyrrole, and then azobisisobutyronitrile (AIBN) as initiator, free radical polymerization; PAP film surface preparation of the PAP method is: PAP Is dissolved in N, N-dimethylformamide (DMF) and then spin-coated onto the gold film; finally, the surface of the film is chemically polymerized with pyrrole in a ferric chloride solution to form a polymerized pyrrole layer. Infrared spectroscopy can be seen, the polymer is the desired product; AFM non-in situ surface topography and three-dimensional topography shows that the coating covers the original gold film surface pores, the surface roughness than pure gold Membrane increased.