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以戴维逊氏棉(Gossypium davidsonii Kell.)叶片切块接种在附加ZT0.1—2mg/l和IAA0.05—1.2mg/l的MS培养基上,培养2—3周,发生愈伤组织。5—6周,在愈伤组织表面出现胚状体,其发生频率达56.5％。将胚状体转移到添加GA_3,IBA和KT的3/4MS或MS培养基中,再生了具有1—8片幼叶的小植株。 细胞学观察表明,胚状体具有结构完整和独立维管束系统的特点,与有性的合子胚相似。还观察到胚状体发生的几个主要过程:从愈伤组织的薄壁细胞产生分生细胞,再形成一圈形成层状细胞包围的小细胞团,继而发育为具有根端(胚根)和茎端(胚芽)的胚状体。
The explants were inoculated with Gossypium davidsonii Kell. Leaves on MS medium supplemented with ZT 0.1-2 mg / l and IAA 0.05-1.2 mg / l and cultured for 2-3 weeks to induce callus . Embryoid bodies appeared on the surface of callus for 5-6 weeks, with a frequency of 56.5%. Embryoid bodies were transferred to 3/4 MS or MS medium supplemented with GA_3, IBA and KT, and plantlets with 1-8 young leaves were regenerated. Cytological observations show that embryoid bodies have the characteristics of structural integrity and independent vascular system, similar to sexual zygotic embryos. Several major processes of embryoid body development were also observed: meristematic cells were produced from the parenchyma cells of the callus, followed by the formation of a small circle of cells surrounding the lamellar cells, And embryo-terminal (germ) embryoid bodies.