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背景:鼻咽癌染色体1p末端结构异常与鼻咽癌发生发展及预后的相关性目前仍然不清楚。目的:探讨鼻咽癌(nasopharyngealcarcinoma,NPC)染色体1p末端(1pter)结构异常与鼻咽癌发生发展及患者部分临床特征的相关性。设计:以诊断为依据,设立对照组的回顾性研究。地点和对象:实验由中山医科大学肿瘤防治实验研究部收集完成,研究对象为中山医科大学肿瘤医院就诊或住院的未经治疗的65例鼻咽癌患者鼻咽活检组织标本。干预:采用组织中淋巴细胞分离法获取较纯的肿瘤细胞,应用1号染色体短臂端粒相关探针(TelVysion1p)及端粒连锁微卫星位点D1S243分别对65例NPC活检组织进行间期荧光原位杂交(fluorescentinsituhy-bridization,FISH)及杂合性缺失(lossofheterozygosity,LOH)和微卫星体不稳定(microsatelliteinstability,MI)分析。主要观察指标:观察鼻咽癌染色体1p末端的缺失、扩增及微卫星体不稳定等结构异常情况及其与临床特征的关系。结果:65例NPC活检组织中,间期FISH显示1pter结构异常的有20例,其中扩增7例,缺失8例,扩增缺失共有5例,其中44例有临床分期资料的标本中,临床早期的异常频率为50.0%(2/4),而中晚期则为30.0%(12/40),两者比较差异无显著性意义(χ2=0.0655,P>0.05);31例NPC活检组织端粒连锁微卫星位点D1S243的
BACKGROUND: The relationship between the structural abnormalities of the 1p end of nasopharyngeal carcinoma chromosome and the occurrence, development and prognosis of nasopharyngeal carcinoma is still unclear. Objective: To investigate the relationship between structural abnormalities of chromosome 1p (NPC) in nasopharyngeal carcinoma (NPC) and the occurrence and development of nasopharyngeal carcinoma and some clinical features of patients. Design: Based on diagnosis, a retrospective study was set up in the control group. Location and Subjects: The experiment was collected by the Experimental Research Department of Cancer Prevention and Treatment of Sun Yat-sen University for the treatment of nasopharyngeal biopsy specimens of 65 patients with nasopharyngeal carcinoma who were treated or hospitalized in Cancer Hospital of Sun Yat-sen University. Intervention: The pure neoplastic cells were obtained by lymphocyte separation in tissues. The telomerase-associated probe (TelVysion1p) on chromosome 1 and the telomere-linked microsatellite locus D1S243 were used to perform the interphase fluorescence In situ hybridization (FISH) and loss of heterozygosity (LOH) and microsatellite instability (MI) analysis were performed. MAIN OUTCOME MEASURES: To observe the structural abnormalities such as deletion, amplification and microsatellite instability of chromosome 1p at the end of nasopharyngeal carcinoma and their relationship with clinical features. Results: Among the 65 cases of NPC biopsy, interphase FISH showed abnormal structure of 1 pter in 20 cases, of which 7 cases were amplified, 8 cases were deleted and 5 cases were amplified and deleted. Of the 44 cases with clinical staging data, clinical The frequency of early abnormalities was 50.0% (2/4), while it was 30.0% (12/40) in the middle and late stages. There was no significant difference between the two groups (χ2 = 0.0655, P> 0.05) Granulo-linked microsatellite locus D1S243