论文部分内容阅读
目的:探讨人源N-myc下游调节基因2(N-Myc downstream-regulated gene 2,NDRG2)小干扰RNA(small interfering RNA,siRNA)对宫颈癌Hela细胞的化疗增敏作用。方法:利用化学合成的针对NDRG2特异性siRNA借助脂质体转染宫颈癌Hela细胞。采用RT-PCR和蛋白质印迹法检测NDRG2mRNA和蛋白在宫颈癌Hela细胞的表达情况。通过MTT法检测该细胞在顺铂作用下的体外存活率。结果:化学合成的NDRG2特异性siRNA oligomer可使Hela细胞的NDRG2表达水平明显降低,增强了顺铂对Hela细胞体外生存的抑制能力。转染Negative-control oligomer的Hela细胞与转染NDRG2siRNA oligomer HSS126269的Hela细胞相比,其存活情况出现明显差异,IC50值分别为(6.69±0.33)和(1.69±0.25)μg/L,差异有统计学意义,t=17.311,P=0.003。结论:抑制NDRG2表达可以提高宫颈癌Hela细胞对顺铂的化疗敏感性,具有一定的临床应用前景。
Objective: To investigate the chemosensitization effect of N-Myc downstream-regulated gene 2 (NDRG2) small interfering RNA (siRNA) on cervical cancer Hela cells. Methods: HeLa cells were transfected with lipofectamine by using chemically synthesized NDRG2-specific siRNA. The expression of NDRG2 mRNA and protein in cervical cancer Hela cells was detected by RT-PCR and Western blotting. The viability of the cells in vitro was tested by MTT assay. Results: The NDRG2-specific siRNA oligomer chemically synthesized could significantly decrease the expression of NDRG2 in Hela cells and enhance the inhibitory effect of cisplatin on the viability of Hela cells in vitro. The survival of Hela cells transfected with Negative-control oligomer was significantly lower than that of Hela cells transfected with NDRG2 siRNA oligomer HSS126269 with IC50 values of (6.69 ± 0.33) and (1.69 ± 0.25) μg / L, respectively Significance, t = 17.311, P = 0.003. Conclusion: Inhibition of NDRG2 expression can improve the chemosensitivity of Cisplatin to cervical cancer Hela cells, and has certain clinical application prospects.