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目的:了解洪灾区钩端螺旋体宿主动物的带菌情况及人群的免疫水平。方法:应用柯素夫培养法作牛尿和鼠肾钩体培养。显微凝集试验(microscopic agglutination test,MAT)作钩体及人群钩体感染的血清学分群(型)。结果:灾区、临灾区及新建移民点的鼠密度分别为6.95%,6.28%及8.67%;鼠肾钩体培养阳性率分别为4.63%,1.35%和3.13%;牛尿培养阳性率分别为5.88%,5.98%和1.75%;分离的菌株主要为黄疸出血型及犬型;人群钩体抗体阳性率分别为45.91%,62.30%和58.67%,明显高于全省及全国的平均感染率;其主要血清学分型依次为黄疸出血、秋季热、犬型、泼摩那型、巴达维亚型;人群抗体阳性率无年龄差异。结论:3个不同区域的宿主动物鼠和牛中均有钩体带菌,人群钩体隐性感染率水平较高,宿主动物和人群感染的主要血清群相吻合,主要为黄疸出血型。
OBJECTIVE: To understand the bacteriological characteristics of Leptospira host organisms and their immunity in flood-affected areas. Methods: Koziff culture method was used for the culture of calf urine and mouse kidney. Microscopic agglutination test (MAT) is used as a serological grouping for leptospira and leptospira infection. Results: The rodent densities of the affected areas were 6.95%, 6.28% and 8.67%, respectively. The positive rates of leptospirosis were 4.63%, 1.35% and 3.13%, respectively. The positive rates of bovine urine culture were 5.88 %, 5.98%, and 1.75%, respectively. The isolates were mainly hemorrhagic and canine types of jaundice. The positive rate of antibody against human leptospira was 45.91%, 62.30% and 58.67% respectively, which was significantly higher than that of the province and the whole country. The main serological types were followed by hemorrhagic jaundice, autumn heat, canine type, splashes type, Batavia type. There was no age difference in antibody positive rate among the population. CONCLUSION: The host animals and cattle in three different regions all have the leptospira-carrying bacteria. The prevalence of latent infection in the leptospira is high. The main serogroups infected by the host animals and the population are consistent, mainly the hemorrhagic jaundice.