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树突状细胞(DC)是一类能特异性启动T淋巴细胞介导的免疫反应的抗原提呈细胞,与其它抗原提呈细胞不同的是,树突状细胞能激活初始型T淋巴细胞(naive T cells).近年来,人们试图通过刺激血液中的前体细胞以提高DC的量.最近,有学者通过GM-CSF、IL-4及MCM的培养单个核细胞中获得了DC.本研究对此法加以改良并从恒河猴血中获得了形态、表型及功能均与人成熟DC非常类似的DC.去除了T细胞的单个核细胞在含有1%人血清及GM-CSF和IL-4的培养基中培养,在第7天,50%的培养基换成单核细胞的条件培养基MCM.此培养基是获得成熟的具有免疫刺激作用的DC所必需的,这样可从2Oml血中获得0.5-1.0×10~6DC.对这些DC与在同样培养基中培养产生的巨噬细胞的比较表明,这些DC刺激同种异体T细胞的能力较巨噬细胞明显增强,而且这些DC的形态和表型很典型且能表达高水平的p55及与细胞内CD68.对比之下,贴壁的巨噬细胞表达非常低水平的p55及高水平的弥漫性CD68,用此方法制备的恒河猴
Dendritic cells (DCs) are a class of antigen-presenting cells that specifically initiate T lymphocyte-mediated immune responses. Unlike other antigen-presenting cells, dendritic cells activate naive T lymphocytes naive T cells. In recent years, attempts have been made to increase the amount of DC by stimulating progenitor cells in the blood.Recently, DCs have been obtained from cultured mononuclear cells by GM-CSF, IL-4 and MCM. This method was modified and DCs were obtained from rhesus monkey blood that were morphologically, phenotypically and functionally similar to human mature DCs. T cell depleted mononuclear cells were cultured in DMEM containing 1% human serum and GM-CSF and IL -4 medium and 50% of the medium was changed to monocyte conditioned medium MCM on day 7. This medium is required for obtaining mature immunostimulatory DCs, so that the medium can be extracted from 2 Oml The blood obtained 0.5-1.0 × 10 ~ 6DC. Comparison of these DC with macrophages cultured in the same medium showed that these DCs stimulate allogeneic T cells than macrophages significantly enhanced ability, and these DC The morphology and phenotype are typical and can express high levels of p55 and intracellular CD68. , Adherent macrophages express very low levels of p55 and high level of CD68 diffuse, rhesus prepared by this method