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目的对比分析4℃静置冷藏保存血小板与22℃振荡保存血小板在不同保存条件下的血小板计数及形态学变化,为研究制定冷藏保存血小板技术提供实验室依据。方法对4℃静置冷藏保存的手工汇集浓缩血小板(实验组)于保存到d1、3、5、7、10、14、21的7个时间点和22℃振荡保存的手工汇集浓缩血小板(对照组)于保存到d1、3、5、7、10的5个时间点进行血样采集,观察对比2组血小板常规计数、瑞斯染色涂片及血小板扫描电子显微镜检测结果。结果 4℃静置冷藏保存手工汇集浓缩血小板21 d内和22℃振荡保存5 d内,血小板计数变化均不明显(P>0.05)。22℃振荡保存手工汇集浓缩血小板的MPV和PDW值5 d内组间差异有统计学意义,呈现增大趋势(P<0.05),4℃冷藏保存手工汇集浓缩血小板14 d内MPV及PDW无明显变化(P>0.05)。瑞斯染色涂片显示,4℃冷藏保存手工汇集浓缩血小板保存到21 d血小板形态、大小变化不明显,血小板胞质致密均匀、着色较深,形态不规则、多数近圆形、大小不等,有少许血小板聚集。22℃振荡保存手工汇集浓缩血小板显示血小板胞质稀松、着色较浅,数量较少。扫描电镜下4℃冷藏保存到10 d时,血小板明显活化、聚集成团,血小板表面凹凸不平,有明显的长伪足形成。而22℃振荡保存到7、10 d时血小板数量较少、有聚集、部分周围出现空晕,形态近圆形,无明显伪足、活化不明显。结论 4℃冷藏保存手工汇集浓缩血小板10-14 d与22℃振荡保存手工汇集浓缩血小板5 d相比,其血小板计数、细胞形态、血小板膜及胞质结构均优于22℃振荡保存。
OBJECTIVE: To compare and analyze the platelet counts and morphological changes of platelets frozen at 4 ℃ and platelets preserved at 22 ℃ under different storage conditions, and provide a laboratory basis for the development of cryopreserved platelet technology. Methods The manual collection of concentrated platelets (experimental group) kept at 4 ℃ for storage at 7 days at d 1, 3, 5, 7, 10, 14 and 21 and manual collection of concentrated platelets by shaking at 22 ℃ The blood samples were collected at 5 time points d 1, 3, 5, 7 and 10, and the routine plate count, Ruiss staining and platelet scanning electron microscopy were compared. Results The platelet count changes were not obvious (P> 0.05) within 4 days at 4 ℃, stored in platelets for 21 days and stored at 22 ℃ for 5 days. MPV and PDW values of manual collection of concentrated platelets at 22 ° C were significantly different within 5 days (P <0.05). There was no significant difference in MPV and PDW within 14 days Change (P> 0.05). Reese staining smears showed that the platelets stored at 4 ℃ were stored in platelets for 21 days, the changes in size were not obvious, the cytoplasm of platelets was dense and uniform, the coloration was darker, the morphology was irregular, most of them were round and the sizes were different. A little platelet aggregation. 22 ℃ oscillation Save manual collection of concentrated platelets showed platelet cytoplasmic sperm, lighter colored, smaller number. Under the scanning electron microscope (SEM), the platelets were activated and stored at 4 ℃ for 10 days. The platelets aggregated into clusters, and the surface of platelets was uneven with obvious pseudopodia formation. However, when the plate was stored at 22 ℃ for 7 and 10 d, the number of platelets was small, aggregated, some of them appeared empty halo, the morphology was nearly circular, no obvious pseudopodia, and the activation was not obvious. Conclusions The platelet counts, cell morphology, platelet membrane and cytoplasmic structure of platelets stored by hand at 4 ℃ for 10-14 days were better than those stored at 22 ℃ by shaking for 10-14 days.