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利用臭氧FACE(Free-Air Ozone Concentration Enrichment)实验平台,研究4个小麦品种“扬麦15”、“扬麦16”、“扬幅麦2号”、“烟农19”根际细菌在拔节期和成熟期对臭氧浓度升高的响应;采用变性梯度凝胶电泳(DGGE)和荧光定量PCR(real-time quantitative PCR)方法,分别对细菌种群结构和数量进行了测定。结果表明:臭氧浓度升高仅显著降低了拔节期“扬幅麦2号”小麦品种根际细菌数量,对成熟期各品种小麦根际细菌数量影响不显著;多样性指数和主成分分析表明,“扬麦15”和“扬幅麦2号”根际细菌多样性和种群结构分别在拔节期和成熟期发生明显变化;臭氧对“扬幅麦2号”品种根际细菌种群的影响较持久,可能会产生累积效应。
Using the Free-Air Ozone Concentration Enrichment (FACE) experimental platform, the effects of four wheat cultivars including “Yangmai 15”, “Yangmai 16”, “Yangmai 2”, “ ”The response of rhizobacteria to the increase of ozone concentration at the jointing and maturing stages; the population structure and number of bacteria were determined by denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR . The results showed that the increase of ozone concentration only significantly reduced the number of rhizosphere bacteria at the jointing stage and the variety of rhizosphere bacteria at the maturity stage. The diversity index and principal component analysis The results showed that rhizosphere bacteria diversity and population structure changed obviously at jointing stage and mature stage respectively in “Yangmai 15” and “Yangmai 2”. Ozone had no significant effect on “Yangmai 2” The rhizospheric bacterial population has a more lasting effect and may have a cumulative effect.