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复方止咳冲剂由甘草、金银花等十几味中药与大量赋形剂制成。甘草酸与绿原酸是其中重要的化学组分。本文建立了同时测定甘草酸与绿原酸的RP-HPLC-UV方法。用C_18不锈钢柱为色谱柱,以甲醇(0.5%醋酸)-水(0.5%醋酸)为流动相,梯度法洗脱,用外标法定量,测定了三批样品.回收率均大于90%,相对标准偏差均小于3%。
Compound cough granules from licorice, honeysuckle and other Chinese medicine and a large number of excipients made. Glycyrrhizic acid and chlorogenic acid are important chemical components. This article established a simultaneous determination of glycyrrhizic acid and chlorogenic acid RP-HPLC-UV method. C_18 stainless steel column was used as the column, and methanol (0.5% acetic acid) - water (0.5% acetic acid) was used as the mobile phase. The gradient elution method was used. Recoveries were greater than 90%, the relative standard deviation were less than 3%.