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目的:探讨叶绿素铜钠促进免疫介导再生障碍性贫血(aplastic anemia,AA)小鼠骨髓间充质干细胞(mensenchymal stem cells,MSC)对T淋巴细胞的干预作用。方法:建立免疫介导AA小鼠模型,随机分为正常对照组(N)、AA模型组(M)、环孢菌素组(Cs)、叶绿素铜钠小剂量组(X)、叶绿素铜钠中剂量组(Z)、叶绿素铜钠高剂量组(G)6组,每组6只,15日后处死,观察各组的骨髓病理,骨髓进行MSC培养,第3代MSC诱导成骨细胞,观察光镜下第2代MSC(F2MSC)、成骨细胞的形态学变化,MSC调节植物血凝素(PHA)对T淋巴细胞转化实验,ELISA检测MSC的转化生长因子(TGF-β1)。结果:N、Cs、X、Z、G组的骨髓增生均比M组活跃。各组F2MSC细胞呈长梭形,第3代MSC可被诱导为成骨细胞。N、X、Z、G组的CD2+5FOXP3+明显大于M组(P<0.05)。N、Cs、Z组的TGF-β1显著大于M组(P<0.05)。结论:叶绿素铜钠能促进AA小鼠MSC对T淋巴细胞的干预作用。
AIM: To investigate the effects of sodium copper brassinide on T lymphocyte mediated by immune-mediated mouse mesenchymal stem cells (MSCs) from aplastic anemia (AA) mice. Methods: The mouse model of immune-mediated AA was established and randomly divided into normal control group (N), AA model group (M), cyclosporine group (Cs), chlorophyll copper sodium low dose group Medium dose group (Z), sodium copper chlorophyll high dose group (G) 6, each group 6, after 15 days were sacrificed to observe the bone marrow pathology in each group, MSC were cultured in the bone marrow, the third generation of MSC induced osteoblasts, observed Morphological changes of the second generation of MSCs (F2MSC) and osteoblasts were observed under the light microscope. MSCs were transformed into PHA for T lymphocyte transformation, and TGF-β1 was detected by ELISA. Results: The bone marrow hyperplasia of group N, Cs, X, Z, G were more active than M group. Each group of F2MSC cells were spindle-shaped, and the third-generation MSCs could be induced to osteoblasts. CD2 + 5 FOXP3 + in group N, X, Z, G was significantly higher than that in group M (P <0.05). TGF-β1 in N, Cs and Z groups was significantly higher than that in M group (P <0.05). Conclusion: Sodium copper chlorophyll can promote the intervention of MSC in T lymphocytes in AA mice.