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目的:研究三七细胞悬浮培养过程中皂苷生物合成的动力学变化。方法:将三七愈伤组织进行悬浮培养40 d,用HPLC检测细胞生长过程中皂苷的动态变化。结果:三七愈伤组织悬浮培养生物量增长缓慢,但检测到6种皂苷的含量随培养时间而变化,人参皂苷Rg1、Re、Rh1、Rb1初始培养值分别为3.63、2.34、0.42、0.24 mg/g,培养25～30 d后增至最高,分别为9.72、7.02、2.37、1.86 mg/g,而Rh2、F1含量无明显变化,即三七愈伤组织生物合成过程中原人参三醇型皂苷(Rg1、Re和Rh1)增长显著。结论:三七愈伤组织培养25 d,总皂苷含量达到最高20.58mg/g,主要为原人参三醇型皂苷。
Objective: To study the dynamic changes of saponin biosynthesis during the suspension culture of Panax notoginseng. Methods: Panax notoginseng callus was cultured in suspension for 40 days. The changes of saponin during cell growth were detected by HPLC. Results: The biomass of suspension culture of Panax notoginseng increased slowly. However, the content of six saponins detected varied with the culture time. The initial culture values of Ginsenoside Rg1, Re, Rh1 and Rb1 were 3.63, 2.34, 0.42 and 0.24 mg / g, and reached the peak after culturing for 25-30 days, which were 9.72, 7.02, 2.37 and 1.86 mg / g, respectively, while the content of Rh2 and F1 did not change significantly, that is, the protopanaxan saponin in the process of Panax notoginseng callus biosynthesis (Rg1, Re and Rh1) increased significantly. Conclusion: The Panax notoginseng callus cultured for 25 days, the total saponin content reached the highest 20.58mg / g, mainly for the original ginseng saponin.