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用绿脓杆菌波动潜生体,羧苄西林诱导潜生体和繁殖体进行抗生素最小抑菌浓度(MIC)试验。将奇异变形杆菌和大肠杆菌pHB101接种于含氨苄西林(AMP)的波动培养基上,动态测定培养基中AMP变化。观察绿脓杆菌波动生长和奇异变形杆菌寄宿波动中细菌对抗生素MIC的不确定性现象并探讨其产生机制。结果表明:绿脓杆菌波动潜生体、羧苄西林诱导潜生体的MIC值比繁殖体高8~16倍。在寄宿波动中,奇异变形杆菌能在高于自己MIC值的AMP波动培养基上向外扩散,波动出两环。因此,细菌由于经历不同,生理状态也不同,因而对抗生素的MIC值表现出不确定性。
Pseudomonas aeruginosa fluctuating latent body, carbenicillin-induced latent body and propagule antibiotic minimum inhibitory concentration (MIC) test. Proteus mirabilis and Escherichia coli pHB101 were inoculated on a fluctuating medium containing ampicillin (AMP), and the AMP change in the medium was measured dynamically. To observe the uncertainty of the antibiotic MIC of bacteria in the fluctuation of Pseudomonas aeruginosa and the boarding fluctuation of Proteus mirabilis, and to explore its mechanism. The results showed that MIC value of carbenicillin-induced latent adult body was 8 ~ 16 times higher than that of propagated body in fluctuating latent body of Pseudomonas aeruginosa. In boarding fluctuations, Proteus mirabilis can spread outwards over AMP-fluctuating medium above its MIC value, fluctuating out of two loops. Therefore, the bacterium has different physiological states due to its different experiences, and thus the MIC value of antibiotics shows uncertainty.