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本研究对不同周数(6、7、8、9、10周)妊娠妇女胎盘绒毛滋养层组织水溶性蛋白质组分、分子量、等电点及其特性进行了分析和比较。用SDS-聚丙烯酰胺凝胶电泳分析结果指明,妊娠早期绒毛组织,除分泌绒毛膜促性腺激素(hCG)这一含量较多的蛋白以外,尚含有其他四种含量较高的蛋白质,这四种蛋白质的分子量分别为:140,000,128,000,94,000和83,000左右。用高压液相色谱层析和等电聚焦电泳分析结果指明,在不同周龄的组织中其蛋白量和组分间的比例稍有变化,较多的蛋白质为酸性蛋白,与蜕膜组织蛋白质组分作比较,绒毛组织具有蜕膜组织内不存在的蛋白组分,其等电点分别为:8.4、6.4、5.8、4.8。由此可知早期妊娠胎盘绒毛滋养层组织,存在一些含量较高的特殊蛋白,深入研究这些蛋白的特性和生理功能,将对阐明人类妊娠生理和寻找控制生育新途径有重要意义。
In this study, we analyzed and compared water-soluble protein components, molecular weight, isoelectric point and their characteristics of placental trophoblast in different gestational weeks (6, 7, 8, 9, 10 weeks) SDS-polyacrylamide gel electrophoresis analysis showed that, in early pregnancy villi, in addition to the secretion of chorionic gonadotropin (hCG), a more content of the protein, it contains four other high protein content, the four The molecular weights of the proteins are: 140,000, 128,000, 94,000 and 83,000 respectively. High-pressure liquid chromatography and isoelectric focusing electrophoresis analysis showed that in different weeks of age the protein content and the ratio between components slightly changed, more acidic protein protein, and decidual tissue proteome As a comparison, villi tissue with decidual tissue does not exist in the protein components, the isoelectric point were: 8.4,6.4,5.8,4.8. This shows that early pregnancy placental trophoblast trophoblast, there are some high content of special proteins, in-depth study of the characteristics of these proteins and physiological functions, will clarify human pregnancy physiology and find new ways to control fertility are of great significance.