论文部分内容阅读
目的:建立HPLC同时测定赤芝药材中灵芝酸C2、灵芝烯酸A、灵芝酸A和灵芝酸D含量的方法。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.03%磷酸水溶液采用梯度洗脱,流速1.0 mL.min-1,检测波长252 nm,柱温35℃。结果:灵芝酸C2、灵芝烯酸A、灵芝酸A和灵芝酸D的线性范围分别为:5.0~50.0 mg.L-1(r=0.999 9),7.2~72 mg.L-1(r=0.999 9),11.67~116.7 mg.L-1(r=0.999 9)和5.32~53.2 mg.L-1(r=0.999 8);平均回收率(n=3)分别为98.8%(RSD1.5%),99.1%(RSD1.9%),99.5%(RSD1.4%)和98.5%(RSD1.9%)。结论:该方法简便、准确,重复性好,为赤芝药材的质量控制提供了实验依据。
OBJECTIVE: To establish a method for the simultaneous determination of Ganoderma lucidum C2, Ganoderma acid A, Ganoderma acid A and Ganoderma acid D in Ganoderma lucidum by HPLC. METHODS: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.03% phosphoric acid solution with a gradient of 1.0 mL · min-1 at a detection wavelength of 252 nm and a column temperature of 35 ℃. Results: The linear ranges of Ganoderma acid C2, Ganoderma acid A, Ganoderma acid A and Ganoderma acid D were 5.0 ~ 50.0 mg.L-1 (r = 0.999 9), 7.2 ~ 72 mg.L-1 (r = 0.999 9), 11.67-116.7 mg.L-1 (r = 0.999 9), and 5.32-53.2 mg.L-1 (r = 0.999 8). The average recoveries (n = 3) were 98.8% %), 99.1% (RSD 1.9%), 99.5% (RSD 1.4%) and 98.5% (RSD 1.9%). Conclusion: The method is simple, accurate and reproducible. It provides an experimental basis for the quality control of Ganoderma lucidum.