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目的探讨丙酮酸乙酯(EP)对氯气染毒大鼠肺组织中一氧化氮(NO)含量及一氧化氮合酶(NOS)活性的影响。方法 30只SD大鼠随机分为正常对照组,染毒组和EP治疗组,每组各10只动物。正常对照组以空气为对照。染毒组给予2 536 mg/m的氯气动态染毒,染毒时间为20 min。染毒后立即给予生理盐水对照或EP(40 mg/kg)治疗。于染毒后6 h麻醉后采集样本进行肺组织病理检测,并测定组织匀浆中NO含量以及总一氧化氮合酶(TNOS)和诱导型一氧化氮合酶(iNOS)活性。结果氯气中毒后病理检查结果显示肺组织呈急性肺损伤(ALI)表现,肺组织中NO含量以及总TNOS和iNOS活性显著增加。EP治疗后显著改善了氯气中毒导致的ALI,降低了肺组织中NO含量以及总TNOS和iNOS活性。结论 EP治疗可以有效缓解氯气中毒导致的ALI,这可能与其抑制NOS活性,降低NO含量有关。
Objective To investigate the effects of ethyl pyruvate (EP) on the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) in the lungs of rats exposed to chlorine. Methods Thirty SD rats were randomly divided into normal control group, exposure group and EP treatment group, with 10 animals in each group. The normal control group with air as a control. The exposure group was given 2 536 mg / m chlorine dynamic exposure, the exposure time was 20 min. Immediately after exposure to saline control or EP (40 mg / kg) treatment. Samples were collected at 6 h after exposure for histopathological examination. The content of NO and the activities of total nitric oxide synthase (TNOS) and inducible nitric oxide synthase (iNOS) were also determined. Results The results of pathological examination after chlorine poisoning showed that lung tissue showed acute lung injury (ALI), NO content in lung tissue and total TNOS and iNOS activity increased significantly. EP treatment significantly improved ALI induced by chlorine poisoning, decreased NO content in lung tissue and total TNOS and iNOS activity. Conclusion EP treatment can effectively alleviate the ALI caused by chlorine poisoning, which may be related to its inhibition of NOS activity and reduce the content of NO.