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研究了血小板激活因子（ＰＡＦ）对兔血小板聚集，牛脑微血管平滑肌细胞ＤＮＡ合成及增殖的影响及四氢吡喃类药物ｔｒａｎｓ－２，６－ｂｉｓ－（３，４－ｄｉｍｅｔｈｏｘｙ－ｐｈｅｎｙｌ）－ｔｅｔｒａｈｙｄｒｏ－（４Ｈ）ｐｙｒａｎ（ＳＺ－１）和ｔｒａｎｓ－２－（３，４，５－ｔｒｉｍｅｔｈｏｘｙｐｈｅｎｙｌ）－６－（２，４－ｄｉｆｌｕｏ－ｒｏｐｈｅｎｙｌ）－ｔｅｔｒａｈｙｄｒｏ－（４Ｈ）ｐｙｒａｎ（ＤＦＴＭ）的拮抗作用．结果表明：ＰＡＦ强烈刺激兔血小板聚集，在１．９１μｍｏｌ·Ｌ－１时，刺激的百分率为７１．７％．ＳＺ－１和ＤＦＴＭ剂量依赖性地抑制ＰＡＦ刺激的兔血小板聚集，ＩＣ５０分别为０．３９和０．８４ｎｍｏｌ·Ｌ－１．ＰＡＦ还刺激牛脑微血管平滑肌细胞增殖，在０．１ｎｍｏｌ·Ｌ－１时作用４８ｈ达最大效应．ＳＺ－１和ＤＦＴＭ显著抑制血小板激活因子的上述作用，在１ｎｍｏｌ·Ｌ－１时抑制率分别为２５．９％和３０．７％．ＳＺ－１和ＤＦＴＭ还抑制ＰＡＦ刺激的牛脑微血管平滑肌细胞ＤＮＡ合成，在１ｎｍｏｌ·Ｌ－１时抑制率分别为２９．１％和２４．４％．实验结果表明：ＰＡＦ可能通过促进血小板聚集，刺激脑血管平滑肌细胞增殖及ＤＮＡ合成而参与?
The effects of platelet activating factor (PAF) on platelet aggregation, DNA synthesis and proliferation in rat brain microvascular smooth muscle cells and the effect of trans-2,6-bis- (3,4-dimethoxy-phenyl) -tetrahydro - (4H) pyran (SZ-1) and trans-2- (3,4,5-trimethoxyphenyl) -6- (2,4-difluo-rophenyl) -tetrahydro- (4H) pyran (DFTM). The results showed that: PAF strongly stimulated rabbit platelet aggregation, the stimulation of the percentage of 71.7% at 1.91μmol·L-1. SZ-1 and DFTM inhibited PAF-stimulated rabbit platelet aggregation dose-dependently with IC50 of 0.39 and 0.84 nmol·L -1, respectively. PAF also stimulated the proliferation of bovine cerebral microvascular smooth muscle cells, and reached the maximal effect at 48 h at 0.1 nmol·L-1. SZ-1 and DFTM significantly inhibited the above-mentioned effects of platelet activating factor, with the inhibition rates of 25.9% and 30.7% at 1 nmol·L-1, respectively. SZ-1 and DFTM also inhibited the DNA synthesis of PAF-stimulated bovine brain microvascular smooth muscle cells, with inhibition rates of 29.1% and 24.4% at 1 nmol·L-1, respectively. The experimental results show that: PAF may participate in promoting platelet aggregation, stimulating the proliferation of vascular smooth muscle cells and DNA synthesis.