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目的:研究新北美圣草苷对淀粉样β蛋白片段25-35(Aβn 25-35)诱导PC12细胞损伤的保护作用，探讨其防治AD的作用机制。n 方法:采用MTT法检测新北美圣草苷对正常PC12细胞及Aβn 25-35损伤PC12细胞增殖的影响，筛选新北美圣草苷溶液的干预浓度。将PC12细胞按随机数字表法分为空白组、模型组、雌二醇组和新北美圣草苷组。空白组与模型组以DMEM培养24 h；雌二醇组加入DMEM和1×10n -3 μmol/L雌二醇溶液；北美圣草苷组加入DMEM和6×10n 4 μmol/L新北美圣草苷溶液，培养2 h后，除空白组外，其余各组加入20 μmol/L的Aβn 25-35储备液，继续培养22 h。采用AnnexinV-FITC/PI双标记检测凋亡率，Western blot法检测雌激素受体β(estrogen receptor β, ERβ)及p-P38/P38蛋白表达，检测PC12细胞上清中乙酰胆碱(acetylcholine, ACh)含量及胆碱乙酰转移酶(Choline acetyl transferase, ChAT)、乙酰胆碱酯酶(Acetylcholin esterase, AChE)活性。n 结果:与模型组比较，新北美圣草苷组细胞凋亡率[(8.080±0.578)%比(18.500±0.870)%]降低(n P<0.01)，ERβ蛋白[(0.348±0.042)比(0.273±0.006)]表达升高(n P<0.01)，p-P38/P38蛋白[(0.372±0.058)比(0.571±0.063)]表达降低(n P<0.01)，ACh[(14.319±1.039)μg/mg比(9.157±1.605)μg/mg]水平及ChAT[(0.715±0.053)U/mg比(0.280±0.093)U/mg]活性增高(n P<0.01)，AChE[(2.607±2.048)U/mg比(6.038±1.867)U/mg]活性降低(n P<0.01)。n 结论:新北美圣草苷可促进Aβn 25-35损伤的PC12细胞增殖，抑制细胞凋亡，具有一定的细胞保护作用，其作用机制可能与调节ERβ表达，抑制P38蛋白磷酸化，提高胆碱能系统功能有关。n “,”Objective:To study the protective effect of neoeriocitrin on PC12 cell injury induced by amyloid β protein fragment 25-35 (Aβ n 25-35), and explore its role in preventing and treating AD.n Methods:The MTT method was used to detect the effect of Neoeriocitrin on the proliferation of normal PC12 cells and Aβn 25-35 damaged PC12 cells, and the intervention concentration of neoeriocitrin solution was screened. The PC12 cells were divided into the normal control group, model group, estradiol group and neoeriocitrin group according to the random number table method. The normal control group and model group were cultured with DMEM for 24 h; DMEM and 1×10n -3 μmol/L estradiol solution were added to the estradiol group; DMEM and 6×10 n 4 μmol/L neoeriocitrin solution were added to the neoeriocitrin group. After 2 hours of culture, except for the normal control group, the remaining groups were added with 20 μmol/L Aβ n 25-35 stock solution, and the culture was continued for 22 h. AnnexinV-FITC/PI double labeling was used to detect apoptosis rate, Western blot method was used to detect estrogen receptor β (estrogen receptor β, ERβ) and p-P38/P38 protein expression. The content of acetylcholine (ACh) was detected, and the activity of Choline acetyl transferase (ChAT) and Acetylcholin esterase (AChE) in the supernatant of PC12 cells were detected.n Results:Compared with the model group, the cell apoptosis rate [(8.080 ± 0.578)% n vs. (18.500 ± 0.870)%] significantly decreased (n P<0.01), the expression of ERβ protein (0.348 ± 0.042n vs. 0.273 ± 0.006) significantly increased (n P<0.01), p-P38/P38 protein expression (0.372 ± 0.058n vs. 0.571 ± 0.063) significantly decreased (n P<0.01), the content of ACh [(14.319 ± 1.039) μg/mgn vs. (9.157 ± 1.605) μg/mg], ChAT activity [(0.715 ± 0.053) U/mg n vs. (0.280 ± 0.093) U/mg] significantly increased (n P<0.01), AChE activity [(2.607 ± 2.048) U/mgn vs. (6.038 ± 1.867) U/mg] significantly reduced (n P<0.01).n Conclusions:Neoeriocitrin can promote the proliferation of PC12 cells damaged by Aβ25-35, inhibit cell apoptosis, and has a certain cytoprotective effect. Its mechanism may be related to the regulation of ERβ expression and inhibition of P38 protein phosphorylation, thereby improving the function of the cholinergic system related.