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[目的]研究食管鳞癌患者癌组织及血浆中DAPK基因甲基化的情况,探讨血浆中DAPK甲基化检测在食管鳞癌早期诊断、治疗、预后评估中的作用。[方法]食管鳞癌患者155例,分别提取血浆、癌组织及癌旁正常组织中DAPK基因的DNA,并结合临床病理以及随访结果,进行DAPK甲基化分析。[结果]癌组织与癌旁正常组织中DAPK的甲基化率差异有统计学意义(P=0.000),癌组织和血浆DAPK基因的甲基化在是否淋巴结转移、是否远处转移、不同分期时差异均有统计学意义(P<0.05)。癌组织和血浆中DAPK基因甲基化的相关系数r为0.849(P<0.000)。癌组织中和血浆中DAPK基因非甲基化患者具有明显的生存优势(P均为0.000)。Cox回归分析显示血浆DAPK甲基化是食管鳞癌患者生存的独立影响因子。[结论]食管鳞癌癌组织和血浆中DAPK基因甲基化均与肿瘤分期、淋巴结转移、远处转移相关;癌组织和血浆中DAPK基因甲基化有显著相关性,血浆中DAPK高甲基化可作为食管鳞癌预后不良的生物标志物。
[Objective] To investigate the methylation status of DAPK gene in cancer tissues and plasma of esophageal squamous cell carcinoma and to explore the role of DAPK methylation detection in the early diagnosis, treatment and prognosis evaluation of esophageal squamous cell carcinoma. [Methods] 155 cases of esophageal squamous cell carcinoma were enrolled in this study. DNA of DAPK gene was extracted from plasma, cancer tissues and adjacent normal tissues respectively. DAPK methylation was analyzed by clinicopathological and follow-up results. [Results] The methylation rates of DAPK in cancer tissues and adjacent normal tissues were significantly different (P = 0.000). The methylation of DAPK in cancer tissues and plasma was significant in lymph node metastasis, distant metastasis and different stages Time difference was statistically significant (P <0.05). The correlation coefficient r of methylation of DAPK gene in cancer tissue and plasma was 0.849 (P <0.000). Patients with non-methylated DAPK gene in the cancerous tissues and in the plasma had a significant survival advantage (P = 0.000). Cox regression analysis showed that plasma DAPK methylation was an independent predictor of survival in esophageal squamous cell carcinoma patients. [Conclusion] Methylation of DAPK gene in esophageal squamous cell carcinoma and plasma is related to tumor stage, lymph node metastasis and distant metastasis. There is a significant correlation between methylation of DAPK gene in plasma and plasma and DAPK hypermethylation in plasma As a poor prognostic biomarkers of esophageal squamous cell carcinoma.