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用放射配基结合法和放免分析法测定了不同ｐＨ值下利多卡因对人血液淋巴细胞β受体结合和肾上腺素刺激的ｃＡＭＰ产量的影响。结果表明，ｐＨ值的增加有利于３Ｈ－ＤＨＡ与β受体结合，肾上腺素刺激的淋巴细胞ｃＡＭＰ的产量随ｐＨ值的增加而增加（Ｐ＜０．００１）；利多卡因抑制β受体结合和肾上腺素刺激的淋巴细胞ｃＡＭＰ产量，其抑制程度亦随ｐＨ值增加而增加（Ｐ＜０．００１）。提示心肺复苏期间不用利多卡因时β受体激动剂的效应随ｐＨ值的增加而增加，如同时使用利多卡因则β受体激动剂的效应并不随ｐＨ值的增加而增强，其机制可能与利多卡因随ｐＨ值的增加，改变了细胞膜脂质流动性，抑制β受体与激动剂的结合有关。
Radioligand binding assay and radioimmunoassay were used to determine the effects of lidocaine on cAMP production by β-adrenoceptor and adrenaline stimulated by human blood lymphocytes at different pH values. The results showed that the increase of pH value was in favor of the binding of 3H-DHA to β receptor, and the production of cAMP in adrenaline-stimulated lymphocytes increased with the increase of pH (P <0.001). Lidocaine inhibited the binding of β receptor And adrenaline-stimulated lymphocyte cAMP production, the degree of inhibition also increased with increasing pH (P <0.001). It suggested that the effect of β-agonist without lidocaine during cardiopulmonary resuscitation increased with the increase of pH value. However, the effect of β-agonist was not enhanced with the increase of pH value when using lidocaine at the same time. The mechanism may be With lidocaine increasing with pH, it changed the lipid mobility of cell membrane and inhibited the binding of β receptor and agonist.