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最近,随着果树矮化栽培技术普及的同时,也开展了矮性砧木大量繁殖的研究。虽然柿子也进行了这方面的研究,但在实际应用中,因柿子插条生根性差,难以通过扦插来大量繁殖苗木,因此采用与苹果、葡萄同样的茎尖组培方法,就成为必然的途径。但以往的研究一直没有成功,这次京都大学农学部果树园艺研究室,在杉浦明教授的指导下获得成功。现将其方法简要介绍如下: 将冬季采取的休眠枝进行冷藏后,在使用时取出,将休眠枝上的茎尖切下,接种
Recently, with the popularity of dwarfing cultivation techniques, the research on the multiplication of dwarf rootstocks has also been carried out. Although persimmons also conducted research in this respect, in practical applications, because of the poor rooting of persimmon cuttings, it is difficult to propagate the nursery stock through cutting. Therefore, it is necessary to adopt the method of tissue culture with tips like apple and grape . However, previous studies have been unsuccessful. The fruit tree horticulture laboratory of Kyoto University Faculty of Agriculture succeeded under the guidance of Prof. Sugimura. The method is now briefly described as follows: the dormant branches taken in the winter to be refrigerated, remove the use of the dormant branches on the dormant stems, inoculation