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目的：探讨间隙连接基因Ｃｘ３２ｍＲＮＡ、Ｃｘ４３ｍＲＮＡ及其蛋白产物在肝细胞癌（ｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａ，ＨＣＣ）发生的重要意义。方法：应用Ｓ－Ｐ免疫组织化学法、原位杂交和流式细胞仪分析技术，研究６１例ＨＣＣ、１４例正常肝组织以及肝癌细胞系ＨＨＣＣ、ＳＭＭＣ－７７２１、正常肝细胞系ＱＺＧ中，Ｃｘ３２ｍＲＮＡ、Ｃｘ４３ｍＲＮＡ及其蛋白产物的表达规律。结果：Ｃｘ３２蛋白在ＨＣＣⅠ、Ⅱ、Ⅲ级和正常肝组织中的阳性率分别为５５．６％、４２．１％、１８．２％和９２．９％；Ｃｘ３２蛋白在ＨＨＣＣ、ＳＭＭＣ－７７２１和ＱＺＧ中阳性细胞计数率分别为１．９％、１．７％和９９．０％。Ｃｘ４３蛋白在ＨＣＣⅠ、Ⅱ、Ⅲ级和正常肝组织中的阳性率分别为４４．４％、２６．３％、１２．１％和７８．６％。Ｃｘ４３蛋白在ＨＨＣＣ、ＳＭＭＣ－７７２１和ＱＺＧ中阳性细胞计数率分别为７．３％、２．３％和９９．１％。Ｃｘ３２、Ｃｘ４３在ＨＣＣ各级和正常肝组织中差异有显著性（Ｐ＜０．０１），在肝癌细胞系ＨＨＣＣ、ＳＭＭＣ－７７２１和正常肝细胞系ＱＺＧ中阳性细胞计数率差异有显著性（Ｐ＜０．０１）。原位杂交显示，Ｃｘ３２ｍＲＮＡ在ＨＣＣⅠ、?
Objective: To investigate the significance of gap junction gene Cx32 mRNA and Cx43 mRNA and their protein products in hepatocellular carcinoma (HCC). Methods: S-P immunohistochemistry, in situ hybridization and flow cytometry were used to investigate the expression of Cx32 mRNA in 61 HCC patients, 14 normal liver tissues and the hepatocellular carcinoma cell lines HHCC, SMMC-7721 and normal liver cell line QZG , Cx43 mRNA and its protein product expression pattern. Results: The positive rates of Cx32 protein in HCC I, II, III and normal liver tissues were 55.6%, 42.1%, 18.2% and 92.9%, respectively. The positive rates of Cx32 protein in HHCC, SMMC-7721 and The positive cell counts in QZG were 1.9%, 1.7% and 99.0%, respectively. The positive rates of Cx43 protein were 44.4%, 26.3%, 12.1% and 78.6% in HCC grade Ⅰ, Ⅱ, Ⅲ and normal liver tissues respectively. The positive cell counts of Cx43 protein in HHCC, SMMC-7721 and QZG were 7.3%, 2.3% and 99.1%, respectively. Cx32 and Cx43 were significantly different between HCCs and normal liver tissues (P <0.01). The positive rate of Cx32 and Cx43 in hepatocellular carcinoma cell lines HHCC, SMMC-7721 and normal liver cell line QZG were significantly different (P <0.01). In situ hybridization showed that Cx32 mRNA was expressed in HCC I