论文部分内容阅读
目的:观察不同剂量含碘中药复方对实验性自身免疫性甲状腺炎(AIT)大鼠的自身抗体、白介素-1、4、6(IL-1、4、6)及肿瘤坏死因子-α(TNF-α)的影响,筛选出治疗AIT中药复方中含碘中药的最佳剂量,并探讨其作用机制。方法:选用普通级SD雌性大鼠75只,取15只为正常组后其余60只进行造模,造模完成后分成4组:模型组、中药低剂量组、中剂量组、高剂量组。酶联免疫法检测各组大鼠的甲状腺抗体,IL-1、4、6,TNF-α水平。结果:中药各治疗组与模型组血清自身抗体,IL-1、4、6及TNF-α比较均有统计学差异(P<0.05),其中中药中、高剂量组与低剂量组比较有统计学差异(P<0.05),中剂量组与高剂量组比较无统计学差异(P>0.05)。结论:中药复方软坚消瘿汤能够通过降低血清自身抗体,IL-1、4、6及TNF-α水平起到有效治疗自身免疫性甲状腺炎的作用,含碘中药海藻、昆布剂量在20～30克之间疗效最佳。
OBJECTIVE: To observe the effects of different doses of iodine-containing traditional Chinese medicine compound on autoantibodies, interleukin-1,4,6 (IL-1,4,6) and tumor necrosis factor-α (TNF) in experimental autoimmune thyroiditis (AIT) -α), screening out the best dose of iodine-containing traditional Chinese medicine compound for treating AIT, and discuss its mechanism. Methods: Seventy five normal SD female rats were selected and 15 rabbits were selected as the normal group. The remaining 60 rabbits were divided into 4 groups: model group, low dose group, medium dose group and high dose group. Thyroid antibodies, IL-1, 4, 6 and TNF-α levels were detected by enzyme-linked immunosorbent assay. Results: The levels of serum autoantibodies, IL-1, IL-4, 6 and TNF-α in Chinese medicine groups were significantly different from those in model group (P <0.05) (P <0.05). There was no significant difference between the middle dose group and the high dose group (P> 0.05). CONCLUSION: Ruanjianxiao Decoction, a traditional Chinese medicine compound, can effectively treat autoimmune thyroiditis by decreasing serum autoantibodies, IL-1, IL-4, 6 and TNF-α levels. The best effect between grams.