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在体外用抗原刺激致敏豚鼠肺气道组织可使其发生收缩反应,加入消炎痛可使这一反应增强。本文观察了消炎痛对致敏豚鼠肺释放白三烯的影响。现将有关资料简介如下。1 材料与方法1.1 豚鼠致敏:豚鼠雌雄不拘,以10％卵清蛋白在后腿肌肉及腹腔各注射1ml,3周后用于实验。1.2 肺组织液的制备:取致敏豚鼠,棒击头部致昏,迅速气管插管与小型动物呼吸机相连,通气,然后开
In vitro sensitization of airway tissue in guinea pigs with sensitized guinea pigs can cause contractile responses, and the addition of indomethacin can increase this response. This article observed the effect of indomethacin on the release of leukotrienes in sensitized guinea pig lungs. The relevant information is now described below. 1 Materials and methods 1.1 Guinea pig sensitization: Guinea pigs are either male or female. 1 ml of ovalbumin was injected into the hind leg muscle and abdominal cavity and used for the experiment after 3 weeks. 1.2 preparation of lung tissue fluid: take sensitized guinea pigs, hit the head to make faint, rapid endotracheal intubation connected to a small animal ventilator, ventilation, and then open