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目的比较紫草素和胡桃醌对舌癌Tca8113细胞的增殖抑制及凋亡诱导作用。方法采用MTT法观察紫草素和胡桃醌对Tca8113细胞的增殖抑制作用并绘制细胞生长曲线;采用Hoechst 33258染色法检测两种药物对Tca8113细胞的凋亡诱导作用;采用SPSS19.0软件包对数据进行单因素方差分析及t检验。结果5~25μmol/L浓度范围内,两种药物对Tca8113细胞的生长抑制作用呈现出明显的剂量和时间依赖性,且两种药物均可诱导Tca8113细胞凋亡;15μmol/L紫草素的增殖抑制作用及诱导凋亡作用均强于胡桃醌(P<0.05)。结论紫草素对Tca8113细胞的增殖抑制作用及凋亡诱导作用强于胡桃醌。
Objective To compare the inhibitory effects of shikonin and juglone on the proliferation of tongue cancer Tca8113 cells and the induction of apoptosis. Methods The inhibitory effect of shikonin and juglone on proliferation of Tca8113 cells was observed by MTT method and the cell growth curve was drawn. The apoptosis induced by two drugs on Tca8113 cells was detected by Hoechst 33258 staining. The data were analyzed by SPSS19.0 software package One-way ANOVA and t test were performed. Results In the concentration range of 5 ~ 25μmol / L, the inhibitory effects of the two drugs on Tca8113 cells were dose-and time-dependent, and both of them could induce the apoptosis of Tca8113 cells. Proliferation of 15μmol / L shikonin Inhibition and induction of apoptosis were stronger than juglone (P <0.05). Conclusion Shikonin can inhibit the proliferation of Tca8113 cells and induce the apoptosis more strongly than juglone.